2ZCQ
Crystal structure of the C(30) carotenoid dehydrosqualene synthase from Staphylococcus aureus complexed with bisphosphonate BPH-652
Summary for 2ZCQ
| Entry DOI | 10.2210/pdb2zcq/pdb |
| Related | 2ZCO 2ZCP 2ZCR 2ZCS |
| Descriptor | Dehydrosqualene synthase, (1R)-4-(3-phenoxyphenyl)-1-phosphonobutane-1-sulfonic acid, MAGNESIUM ION, ... (4 entities in total) |
| Functional Keywords | crtm, carotenoid biosynthesis, staphyloxanthin biosynthesis, transferase, head-to-head condensation, bisphosphonate |
| Biological source | Staphylococcus aureus |
| Total number of polymer chains | 1 |
| Total formula weight | 35222.46 |
| Authors | Liu, C.I.,Jeng, W.Y.,Wang, A.H.,Oldfield, E. (deposition date: 2007-11-11, release date: 2008-03-11, Last modification date: 2023-11-01) |
| Primary citation | Liu, C.I.,Liu, G.Y.,Song, Y.,Yin, F.,Hensler, M.E.,Jeng, W.Y.,Nizet, V.,Wang, A.H.,Oldfield, E. A cholesterol biosynthesis inhibitor blocks Staphylococcus aureus virulence. Science, 319:1391-1394, 2008 Cited by PubMed Abstract: Staphylococcus aureus produces hospital- and community-acquired infections, with methicillin-resistant S. aureus posing a serious public health threat. The golden carotenoid pigment of S. aureus, staphyloxanthin, promotes resistance to reactive oxygen species and host neutrophil-based killing, and early enzymatic steps in staphyloxanthin production resemble those for cholesterol biosynthesis. We determined the crystal structures of S. aureus dehydrosqualene synthase (CrtM) at 1.58 angstrom resolution, finding structural similarity to human squalene synthase (SQS). We screened nine SQS inhibitors and determined the structures of three, bound to CrtM. One, previously tested for cholesterol-lowering activity in humans, blocked staphyloxanthin biosynthesis in vitro (median inhibitory concentration approximately 100 nM), resulting in colorless bacteria with increased susceptibility to killing by human blood and to innate immune clearance in a mouse infection model. This finding represents proof of principle for a virulence factor-based therapy against S. aureus. PubMed: 18276850DOI: 10.1126/science.1153018 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.38 Å) |
Structure validation
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