2XMS
Crystal structure of human NDRG2 protein provides insight into its role as a tumor suppressor
Summary for 2XMS
Entry DOI | 10.2210/pdb2xms/pdb |
Related | 2XMQ 2XMR |
Descriptor | PROTEIN NDRG2, CHLORIDE ION, IMIDAZOLE, ... (4 entities in total) |
Functional Keywords | signaling protein, ndrg family |
Biological source | HOMO SAPIENS (HUMAN) |
Cellular location | Cytoplasm: Q9UN36 |
Total number of polymer chains | 1 |
Total formula weight | 31579.15 |
Authors | |
Primary citation | Hwang, J.,Kim, Y.,Kang, H.B.,Jaroszewski, L.,Deacon, A.,Lee, H.,Choi, W.C.,Kim, K.J.,Kim, C.H.,Kang, B.S.,Lee, J.O.,Oh, T.K.,Kim, J.W.,Wilson, I.A.,Kim, M.H. Crystal Structure of Human Ndrg2 Protein Provides Insight Into its Role as a Tumor Suppressor. J.Biol.Chem., 286:12450-, 2011 Cited by PubMed Abstract: Considerable attention has recently been paid to the N-Myc downstream-regulated gene (NDRG) family because of its potential as a tumor suppressor in many human cancers. Primary amino acid sequence information suggests that the NDRG family proteins may belong to the α/β-hydrolase (ABH) superfamily; however, their functional role has not yet been determined. Here, we present the crystal structures of the human and mouse NDRG2 proteins determined at 2.0 and 1.7 Å resolution, respectively. Both NDRG2 proteins show remarkable structural similarity to the ABH superfamily, despite limited sequence similarity. Structural analysis suggests that NDRG2 is a nonenzymatic member of the ABH superfamily, because it lacks the catalytic signature residues and has an occluded substrate-binding site. Several conserved structural features suggest NDRG may be involved in molecular interactions. Mutagenesis data based on the structural analysis support a crucial role for helix α6 in the suppression of TCF/β-catenin signaling in the tumorigenesis of human colorectal cancer, via a molecular interaction. PubMed: 21247902DOI: 10.1074/JBC.M110.170803 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.15 Å) |
Structure validation
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