2XMS

Crystal structure of human NDRG2 protein provides insight into its role as a tumor suppressor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6C1
Synchrotron site	PAL/PLS
Beamline	6C1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 31 2 1
Unit cell lengths	93.098, 93.098, 90.205
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.150
R-factor	0.1744
Rwork	0.173
R-free	0.20750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qmq
RMSD bond length	0.015
RMSD bond angle	1.463
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.230
High resolution limit [Å]	2.150	2.150
Rmerge	0.080	0.500
Number of reflections	24942
<I/σ(I)>	28.16	2.78
Completeness [%]	99.9	99.8
Redundancy	9.1	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				1.5 M NACL AND 0.1 M IMIDAZOLE (PH 7.0 TO 8.0)