2XI2

HCV-H77 NS5B Apo Polymerase

Summary for 2XI2

• Entry DOI: 10.2210/pdb2xi2/pdb
• Related: 1A1R 1A1V 1CWX 1HEI 1JR6 1N1L 1ONB 1R7C 1R7D 1R7E 1R7F 1R7G 1RGQ 2XI3
• Descriptor: RNA-directed RNA polymerase, SULFATE ION (3 entities in total)
• Functional Keywords: transferase, nonstructural protein, replication, rdrp, de novo priming
• Biological source: Hepatitis C virus genotype 1a (isolate H77) (HCV)
• Cellular location: Core protein p21: Host endoplasmic reticulum membrane; Single-pass membrane protein (By similarity). Core protein p19: Virion (By similarity). Envelope glycoprotein E1: Virion membrane; Single-pass type I membrane protein (Potential). Envelope glycoprotein E2: Virion membrane; Single-pass type I membrane protein (Potential). p7: Host endoplasmic reticulum membrane; Multi-pass membrane protein. Protease NS2-3: Host endoplasmic reticulum membrane; Multi-pass membrane protein (Potential). Serine protease NS3: Host endoplasmic reticulum membrane; Peripheral membrane protein (By similarity). Non-structural protein 4A: Host endoplasmic reticulum membrane; Single-pass type I membrane protein (Potential). Non-structural protein 4B: Host endoplasmic reticulum membrane; Multi-pass membrane protein. Non-structural protein 5A: Host endoplasmic reticulum membrane; Peripheral membrane protein. RNA-directed RNA polymerase: Host endoplasmic reticulum membrane; Single-pass type I membrane protein (Potential): P27958
• Total number of polymer chains: 3
• Total formula weight: 193279.72

Authors

Harrus, D.,Ahmed-El-Sayed, N.,Simister, P.C.,Miller, S.,Triconnet, M.,Hagedorn, C.H.,Mahias, K.,Rey, F.A.,Astier-Gin, T.,Bressanelli, S. (deposition date: 2010-06-25, release date: 2010-08-04, Last modification date: 2024-03-27)

• Primary citation: Harrus, D.,Ahmed-El-Sayed, N.,Simister, P.C.,Miller, S.,Triconnet, M.,Hagedorn, C.H.,Mahias, K.,Rey, F.A.,Astier-Gin, T.,Bressanelli, S.; Further Insights Into the Roles of GTP and the C- Terminus of the Hepatitis C Virus Polymerase in the Initiation of RNA Synthesis; J.Biol.Chem., 285:32906-, 2010; PubMed: 20729191; DOI: 10.1074/JBC.M110.151316

Experimental method

X-RAY DIFFRACTION (1.8 Å)