2XI2
HCV-H77 NS5B Apo Polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2003-01-30 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.332, 272.428, 60.971 |
| Unit cell angles | 90.00, 98.75, 90.00 |
Refinement procedure
| Resolution | 43.053 - 1.800 |
| R-factor | 0.1641 |
| Rwork | 0.163 |
| R-free | 0.20710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1quv |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.006 |
| Data reduction software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.100 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.080 | 0.350 |
| Number of reflections | 144615 | |
| <I/σ(I)> | 13.2 | 1.9 |
| Completeness [%] | 91.9 | 81.6 |
| Redundancy | 2.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | MES 0.1M PH 6.5, AMMONIUM SULFATE 0.2M, PEG 5000 MONOMETHYL ETHER |
