2WZQ

Insertion Mutant E173GP174 of the NS3 protease-helicase from dengue virus

2WZQ の概要

• エントリーDOI: 10.2210/pdb2wzq/pdb
• 関連するPDBエントリー: 2VBC
• 分子名称: NS3 PROTEASE-HELICASE, CHLORIDE ION, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: virion, hydrolase, transmembrane, envelope protein, nucleotide-binding, capsid protein, domain mobility, rna replication
• 由来する生物種: DENGUE VIRUS; 詳細
• 細胞内の位置: Envelope protein E: Virion membrane; Multi- pass membrane protein: Q2TN89 Q2TN89
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 72600.85

構造登録者

Luo, D.,Wei, N.,Doan, D.,Paradkar, P.,Chong, Y.,Davidson, A.,Kotaka, M.,Lescar, J.,Vasudevan, S. (登録日: 2009-12-02, 公開日: 2010-04-07, 最終更新日: 2023-12-20)

主引用文献

Luo, D.,Wei, N.,Doan, D.N.,Paradkar, P.N.,Chong, Y.,Davidson, A.D.,Kotaka, M.,Lescar, J.,Vasudevan, S.G.
Flexibility between the Protease and Helicase Domains of the Dengue Virus Ns3 Protein Conferred by the Linker Region and its Functional Implications.
J.Biol.Chem., 285:18817-, 2010

PubMed Abstract: The dengue virus (DENV) NS3 protein is essential for viral polyprotein processing and RNA replication. It contains an N-terminal serine protease region (residues 1-168) joined to an RNA helicase (residues 180-618) by an 11-amino acid linker (169-179). The structure at 3.15 A of the soluble NS3 protein from DENV4 covalently attached to 18 residues of the NS2B cofactor region (NS2B(18)NS3) revealed an elongated molecule with the protease domain abutting subdomains I and II of the helicase (Luo, D., Xu, T., Hunke, C., Grüber, G., Vasudevan, S. G., and Lescar, J. (2008) J. Virol. 82, 173-183). Unexpectedly, using similar crystal growth conditions, we observed an alternative conformation where the protease domain has rotated by approximately 161 degrees with respect to the helicase domain. We report this new crystal structure bound to ADP-Mn(2+) refined to a resolution of 2.2 A. The biological significance for interdomain flexibility conferred by the linker region was probed by either inserting a Gly residue between Glu(173) and Pro(174) or replacing Pro(174) with a Gly residue. Both mutations resulted in significantly lower ATPase and helicase activities. We next increased flexibility in the linker by introducing a Pro(176) to Gly mutation in a DENV2 replicon system. A 70% reduction in luciferase reporter signal and a similar reduction in the level of viral RNA synthesis were observed. Our results indicate that the linker region has evolved to an optimum length to confer flexibility to the NS3 protein that is required both for polyprotein processing and RNA replication.

PubMed: 20375022
DOI: 10.1074/JBC.M109.090936

実験手法

X-RAY DIFFRACTION (2.8 Å)