2VOH

Structure of mouse A1 bound to the Bak BH3-domain

2VOH の概要

• エントリーDOI: 10.2210/pdb2voh/pdb
• 関連するPDBエントリー: 2VOF 2VOG 2VOI
• 分子名称: BCL-2-RELATED PROTEIN A1, BCL-2 HOMOLOGOUS ANTAGONIST/KILLER, CITRIC ACID, ... (5 entities in total)
• 機能のキーワード: bh3, bcl-2, membrane, apoptosis, pro-survival, transmembrane, protein-protein complex
• 由来する生物種: MUS MUSCULUS (MOUSE); 詳細
• 細胞内の位置: Cytoplasm: Q07440; Mitochondrion membrane; Single-pass membrane protein (Potential): O08734
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 21127.82

構造登録者

Smits, C.,Czabotar, P.E.,Hinds, M.G.,Day, C.L. (登録日: 2008-02-17, 公開日: 2008-03-04, 最終更新日: 2023-12-13)

主引用文献

Smits, C.,Czabotar, P.E.,Hinds, M.G.,Day, C.L.
Structural Plasticity Underpins Promiscuous Binding of the Prosurvival Protein A1.
Structure, 16:818-, 2008

PubMed Abstract: Apoptotic pathways are regulated by protein-protein interactions. Interaction of the BH3 domains of proapoptotic Bcl-2 family proteins with the hydrophobic groove of prosurvival proteins is critical. Whereas some BH3 domains bind in a promiscuous manner, others exhibit considerable selectivity and the sequence characteristics that distinguish these activities are unclear. In this study, crystal structures of complexes between the prosurvival protein A1 and the BH3 domains from Puma, Bmf, Bak, and Bid have been solved. The structure of A1 is similar to that of other prosurvival proteins, although features, such as an acidic patch in the binding groove, may allow specific therapeutic modulation of apoptosis. Significant conformational plasticity was observed in the intermolecular interactions and these differences explain some of the variation in affinity. This study, in combination with published data, suggests that interactions between conserved residues demarcate optimal binding.

PubMed: 18462686
DOI: 10.1016/J.STR.2008.02.009

実験手法

X-RAY DIFFRACTION (1.9 Å)