2VE7

Crystal structure of a bonsai version of the human Ndc80 complex

Summary for 2VE7

• Entry DOI: 10.2210/pdb2ve7/pdb
• Descriptor: KINETOCHORE PROTEIN HEC1, KINETOCHORE PROTEIN SPC25, KINETOCHORE PROTEIN NUF2, KINETOCHORE PROTEIN SPC24, GLYCEROL, ... (5 entities in total)
• Functional Keywords: mitosis, centromere, cell cycle, microtubule, kinetochore, cell division, calponin homology
• Biological source: HOMO SAPIENS (HUMAN); More
• Cellular location: Nucleus: Q9HBM1 Q8NBT2
• Total number of polymer chains: 4
• Total formula weight: 131061.84

Authors

Ciferri, C.,Pasqualato, S.,Dos Reis, G.,Screpanti, E.,Maiolica, A.,Polka, J.,De Luca, J.G.,De Wulf, P.,Salek, M.,Rappsilber, J.,Moores, C.A.,Salmon, E.D.,Musacchio, A. (deposition date: 2007-10-17, release date: 2008-05-13, Last modification date: 2024-06-19)

Primary citation

Ciferri, C.,Pasqualato, S.,Screpanti, E.,Varetti, G.,Santaguida, S.,Dos Reis, G.,Maiolica, A.,Polka, J.,De Luca, J.G.,De Wulf, P.,Salek, M.,Rappsilber, J.,Moores, C.A.,Salmon, E.D.,Musacchio, A.
Implications for Kinetochore-Microtubule Attachment from the Structure of an Engineered Ndc80 Complex
Cell(Cambridge,Mass.), 133:427-, 2008

PubMed Abstract: Kinetochores are proteinaceous assemblies that mediate the interaction of chromosomes with the mitotic spindle. The 180 kDa Ndc80 complex is a direct point of contact between kinetochores and microtubules. Its four subunits contain coiled coils and form an elongated rod structure with functional globular domains at either end. We crystallized an engineered "bonsai" Ndc80 complex containing a shortened rod domain but retaining the globular domains required for kinetochore localization and microtubule binding. The structure reveals a microtubule-binding interface containing a pair of tightly interacting calponin-homology (CH) domains with a previously unknown arrangement. The interaction with microtubules is cooperative and predominantly electrostatic. It involves positive charges in the CH domains and in the N-terminal tail of the Ndc80 subunit and negative charges in tubulin C-terminal tails and is regulated by the Aurora B kinase. We discuss our results with reference to current models of kinetochore-microtubule attachment and centromere organization.

PubMed: 18455984
DOI: 10.1016/J.CELL.2008.03.020

Experimental method

X-RAY DIFFRACTION (2.88 Å)