2VE7

Crystal structure of a bonsai version of the human Ndc80 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	KINETOCHORE PROTEIN HEC1, KINETOCHORE PROTEIN SPC25	polymer	315	36351.3	2	UniProt (O14777) UniProt (Q9HBM1) Pfam (PF03801) Pfam (PF18077) Pfam (PF08234)	HOMO SAPIENS (HUMAN)	NDC80-SPC25 CHIMERA, HSHEC1, KINETOCHORE-ASSOCIATED PROTEIN 2, HIGHLY EXPRESSED IN CANCER PROTEIN, RETINOBLASTOMA-ASSOCIATED PROTEIN HEC, HSPC25
2	C, D (C, D)	KINETOCHORE PROTEIN NUF2, KINETOCHORE PROTEIN SPC24	polymer	250	28993.4	2	UniProt (Q9BZD4) UniProt (Q8NBT2) Pfam (PF03800) Pfam (PF08286)	HOMO SAPIENS (HUMAN)	NUF2-SPC24 CHIMERA, HNUF2, HNUF2R, HSNUF2, CELL DIVISION CYCLE-ASSOCIATED PROTEIN 1, HSPC24
3	E, F (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	G, H (C, D)	PHENOL	non-polymer		94.1	2	Chemie (IPH)
5	I, J, K, L (A, B, C, D)	water	water		18.0	22	Chemie (HOH)

Sequence modifications

A, B: 80 - 286 (UniProt: O14777)
A, B: 1118 - 1224 (UniProt: Q9HBM1)

PDB	External Database	Details
Met 79	-	expression tag
Gln 1224	Asn 224	conflict

C, D: 1 - 169 (UniProt: Q9BZD4)

PDB	External Database	Details
Gly 72	Glu 72	engineered mutation

C, D: 1122 - 1197 (UniProt: Q8NBT2)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Glu 1152	Asp 152	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	130689.4
Non-Polymers*	Number of molecules	4
	Total formula weight	372.4
All*	Total formula weight	131061.8

*Water molecules are not included.