2R7Z

Cisplatin lesion containing RNA polymerase II elongation complex

Summary for 2R7Z

Descriptor5'-D(*TP*AP*CP*TP*TP*GUP*CP*CP*CP*TP*CP*CP*TP*CP*AP*T)-3', 5'-D(*CP*AP*AP*GP*TP*AP*G)-3', 5'-R(*UP*UP*UP*GP*AP*GP*GP*AP*GP*G)-3', ... (18 entities in total)
Functional Keywordstransferase/dna/rna, dna damage, zinc-finger, dna-binding, phosphorylation, cisplatin lesion, misincorporation, rna polymerase ii, transcription- coupled repair, tcr, arrest, stalling, dna lesion, metal-binding, nuclear protein, transcription bubble, damage recognition, elongation complex, transferase, transcription, transferase-dna-rna complex, transcription-dna-rna hybrid complex, transcription/dna-rna hybrid
Biological sourceSaccharomyces cerevisiae (baker's yeast)
Cellular locationNucleus P04050 P08518 P16370 P20433 P20434 P34087 P20436 P38902
Cytoplasm  P20435
Nucleus, nucleolus  P27999 P22139 P40422
Total number of polymer chains15
Total molecular weight525499.57
Authors
Damsma, G.E.,Alt, A.,Brueckner, F.,Carell, T.,Cramer, P. (deposition date: 2007-09-10, release date: 2007-11-20, Last modification date: 2017-10-25)
Primary citation
Damsma, G.E.,Alt, A.,Brueckner, F.,Carell, T.,Cramer, P.
Mechanism of transcriptional stalling at cisplatin-damaged DNA.
Nat.Struct.Mol.Biol., 14:1127-1133, 2007
PubMed: 17994106 (PDB entries with the same primary citation)
DOI: 10.1038/nsmb1314
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (3.8 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone0.2445310.5%8.8%0.7%0.62MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution