2R7Z
Cisplatin lesion containing RNA polymerase II elongation complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-18 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.07155 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 222.062, 393.123, 283.733 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.800 |
Rwork | 0.215 |
R-free | 0.24010 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.020 |
High resolution limit [Å] | 3.800 | 3.800 |
Number of reflections | 235052 | |
<I/σ(I)> | 3.37 | |
Completeness [%] | 31.2 | 32 |
Redundancy | 3.24 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 200 mM ammonium acetate, 150 mM magnesium acetate, 50 mM Hepes pH 7.0, 5% (v/v) PEG 6000, 5 mM TCEP, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | ammonium acetate | ||
2 | 1 | 1 | magnesium acetate | ||
3 | 1 | 1 | Hepes | ||
4 | 1 | 1 | PEG 6000 | ||
5 | 1 | 1 | TCEP | ||
6 | 1 | 2 | ammonium acetate | ||
7 | 1 | 2 | magnesium acetate | ||
8 | 1 | 2 | Hepes | ||
9 | 1 | 2 | PEG 6000 |