2R7Z
Cisplatin lesion containing RNA polymerase II elongation complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.07155 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 222.062, 393.123, 283.733 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.800 |
| Rwork | 0.215 |
| R-free | 0.24010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.020 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Number of reflections | 235052 | |
| <I/σ(I)> | 3.37 | |
| Completeness [%] | 31.2 | 32 |
| Redundancy | 3.24 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 200 mM ammonium acetate, 150 mM magnesium acetate, 50 mM Hepes pH 7.0, 5% (v/v) PEG 6000, 5 mM TCEP, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ammonium acetate | ||
| 2 | 1 | 1 | magnesium acetate | ||
| 3 | 1 | 1 | Hepes | ||
| 4 | 1 | 1 | PEG 6000 | ||
| 5 | 1 | 1 | TCEP | ||
| 6 | 1 | 2 | ammonium acetate | ||
| 7 | 1 | 2 | magnesium acetate | ||
| 8 | 1 | 2 | Hepes | ||
| 9 | 1 | 2 | PEG 6000 |
