2R36

Crystal structure of ni human ARG-insulin

Summary for 2R36

• Entry DOI: 10.2210/pdb2r36/pdb
• Related: 1OS4 2QIU 2R34 2R35
• Descriptor: Insulin, NICKEL (II) ION, SODIUM ION, ... (6 entities in total)
• Functional Keywords: hormone, glucose utilisation, t6 conformation
• Biological source: Homo sapiens (human); More
• Cellular location: Secreted: P01308 P01308
• Total number of polymer chains: 4
• Total formula weight: 12242.90

Authors

Sreekanth, R.,Pattabhi, V.,Rajan, S.S. (deposition date: 2007-08-29, release date: 2008-09-02, Last modification date: 2023-10-25)

Primary citation

Sreekanth, R.,Pattabhi, V.,Rajan, S.S.
Metal induced structural changes observed in hexameric insulin
Int.J.Biol.Macromol., 44:29-36, 2009

PubMed Abstract: The metal ions in insulin hexamer play a crucial role in the T to R conformational transitions. We have determined the crystal structures of 2Mn2+, 1Rb1+ and 4Ni2+ human arg-insulin and compared them with the 2Zn2+ structure. The first two structures exist in the T3R3f state like the native 2Zn2+ arg-insulin, while the 4Ni2+ adopts a T6 conformation. The metal coordination is found to be tetrahedral in all the structures except that of nickel where a dual octahedral and tetrahedral coordination is found at one site. Rubidium occupies only one of the high affinity metal binding sites. The metal induced structural changes observed, have been explained.

PubMed: 18977386
DOI: 10.1016/j.ijbiomac.2008.09.019

Experimental method

X-RAY DIFFRACTION (2 Å)