2R35

Crystal structure of RB human arg-insulin

Summary for 2R35

Entry DOI	10.2210/pdb2r35/pdb
Related	1TRZ 2QIU 2R34 2R36
Descriptor	Insulin, SODIUM ION, RUBIDIUM ION, ... (5 entities in total)
Functional Keywords	hormone, glucose utilisation, t3r3 conformation
Biological source	Homo sapiens (human) More
Cellular location	Secreted: P01308 P01308
Total number of polymer chains	4
Total formula weight	12058.15
Authors	Sreekanth, R.,Pattabhi, V.,Rajan, S.S. (deposition date: 2007-08-29, release date: 2008-09-02, Last modification date: 2024-10-30)
Primary citation	Sreekanth, R.,Pattabhi, V.,Rajan, S.S. Metal induced structural changes observed in hexameric insulin Int.J.Biol.Macromol., 44:29-36, 2009 Cited by PubMed Abstract: The metal ions in insulin hexamer play a crucial role in the T to R conformational transitions. We have determined the crystal structures of 2Mn2+, 1Rb1+ and 4Ni2+ human arg-insulin and compared them with the 2Zn2+ structure. The first two structures exist in the T3R3f state like the native 2Zn2+ arg-insulin, while the 4Ni2+ adopts a T6 conformation. The metal coordination is found to be tetrahedral in all the structures except that of nickel where a dual octahedral and tetrahedral coordination is found at one site. Rubidium occupies only one of the high affinity metal binding sites. The metal induced structural changes observed, have been explained. PubMed: 18977386 DOI: 10.1016/j.ijbiomac.2008.09.019 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.08 Å)

Structure validation