2R35

Crystal structure of RB human arg-insulin

2R35 の概要

• エントリーDOI: 10.2210/pdb2r35/pdb
• 関連するPDBエントリー: 1TRZ 2QIU 2R34 2R36
• 分子名称: Insulin, SODIUM ION, RUBIDIUM ION, ... (5 entities in total)
• 機能のキーワード: hormone, glucose utilisation, t3r3 conformation
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Secreted: P01308 P01308
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 12058.15

構造登録者

Sreekanth, R.,Pattabhi, V.,Rajan, S.S. (登録日: 2007-08-29, 公開日: 2008-09-02, 最終更新日: 2024-10-30)

主引用文献

Sreekanth, R.,Pattabhi, V.,Rajan, S.S.
Metal induced structural changes observed in hexameric insulin
Int.J.Biol.Macromol., 44:29-36, 2009

PubMed Abstract: The metal ions in insulin hexamer play a crucial role in the T to R conformational transitions. We have determined the crystal structures of 2Mn2+, 1Rb1+ and 4Ni2+ human arg-insulin and compared them with the 2Zn2+ structure. The first two structures exist in the T3R3f state like the native 2Zn2+ arg-insulin, while the 4Ni2+ adopts a T6 conformation. The metal coordination is found to be tetrahedral in all the structures except that of nickel where a dual octahedral and tetrahedral coordination is found at one site. Rubidium occupies only one of the high affinity metal binding sites. The metal induced structural changes observed, have been explained.

PubMed: 18977386
DOI: 10.1016/j.ijbiomac.2008.09.019

実験手法

X-RAY DIFFRACTION (2.08 Å)