2PI4

T7RNAP complexed with a phi10 protein and initiating GTPs.

Summary for 2PI4

• Entry DOI: 10.2210/pdb2pi4/pdb
• Related: 2PI5
• Descriptor: 5'-D(*CP*TP*TP*CP*CP*TP*AP*TP*AP*GP*TP*GP*AP*GP*TP*CP*GP*TP*AP*TP*TP*A)-3', 5'-D(*TP*AP*AP*TP*AP*CP*GP*AP*CP*TP*CP*AP*CP*T)-3', DNA-directed RNA polymerase, ... (6 entities in total)
• Functional Keywords: t7 rna polymerase, initiating nucleotides., transferase-dna complex, transferase/dna
• Biological source: Enterobacteria phage T7
• Total number of polymer chains: 3
• Total formula weight: 110429.68

Authors

Kennedy, W.P.,Momand, J.R.,Yin, Y.W. (deposition date: 2007-04-12, release date: 2007-06-19, Last modification date: 2023-08-30)

• Primary citation: Kennedy, W.P.,Momand, J.R.,Yin, Y.W.; Mechanism for de novo RNA synthesis and initiating nucleotide specificity by t7 RNA polymerase.; J.Mol.Biol., 370:256-268, 2007; PubMed: 17512007; DOI: 10.1016/j.jmb.2007.03.041

Experimental method

X-RAY DIFFRACTION (2.5 Å)