2PI0

Crystal Structure of IRF-3 bound to the PRDIII-I regulatory element of the human interferon-B enhancer

2PI0 の概要

• エントリーDOI: 10.2210/pdb2pi0/pdb
• 分子名称: PRDIII-I region of human interferon-B promoter strand 1, PRDIII-I region of human interferon-B promoter strand 2, Interferon regulatory factor 3, ... (4 entities in total)
• 機能のキーワード: interferon, protein-dna complex, transcription, transcription activator-dna complex, transcription activator/dna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm: 2PI0
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 73272.20

構造登録者

Escalante, C.R.,Nistal-Villan, E.,Leyi, S.,Garcia-Sastre, A.,Aggarwal, A.K. (登録日: 2007-04-12, 公開日: 2007-10-30, 最終更新日: 2023-08-30)

主引用文献

Escalante, C.R.,Nistal-Villan, E.,Shen, L.,Garcia-Sastre, A.,Aggarwal, A.K.
Structure of IRF-3 bound to the PRDIII-I regulatory element of the human interferon-beta enhancer.
Mol.Cell, 26:703-716, 2007

PubMed Abstract: Interferon regulatory factor 3 (IRF-3) is a key transcription factor in the assembly of the mammalian interferon-beta (IFN-beta) enhanceosome. We present here the structure of IRF-3 DNA binding domain in complex with the complete PRDIII-I regulatory element of the human IFN-beta enhancer. We show that four IRF-3 molecules bind in tandem to, variably spaced, consensus and nonconsensus IRF sites on the composite element. The ability of IRF-3 to bind these variable sites derives in part from two nonconserved arginines (Arg78 and Arg86) that partake in alternate protein-DNA contacts. We also show that the protein-DNA contacts are highly overlapped and that all four IRF sites are required for gene activation in vivo. In addition, we show that changing the nonconsensus IRF sites to consensus sites creates a more efficient enhancer in vivo. Together, the structure and accompanying biological data provide insights into the assembly of the IFN-beta enhanceosome in mammals.

PubMed: 17560375
DOI: 10.1016/j.molcel.2007.04.022

実験手法

X-RAY DIFFRACTION (2.31 Å)