2PI0
Crystal Structure of IRF-3 bound to the PRDIII-I regulatory element of the human interferon-B enhancer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 109 |
| Detector technology | CCD |
| Collection date | 2006-04-06 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.417, 46.132, 129.325 |
| Unit cell angles | 90.00, 129.42, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.310 |
| R-factor | 0.18519 |
| Rwork | 0.181 |
| R-free | 0.26433 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t2k |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.448 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.027 | 0.055 |
| Number of reflections | 30304 | |
| <I/σ(I)> | 34.8 | 10.8 |
| Completeness [%] | 91.7 | 74.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 12%PEG 400,200mM NaCl, pH 7., VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 400 | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 2 | PEG 400 | ||
| 4 | 1 | 2 | NaCl |
