2PI0

Crystal Structure of IRF-3 bound to the PRDIII-I regulatory element of the human interferon-B enhancer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	109
Detector technology	CCD
Collection date	2006-04-06
Detector	ADSC QUANTUM 210
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	173.417, 46.132, 129.325
Unit cell angles	90.00, 129.42, 90.00

Refinement procedure

Resolution	50.000 - 2.310
R-factor	0.18519
Rwork	0.181
R-free	0.26433
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t2k
RMSD bond length	0.022
RMSD bond angle	2.448
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.310	2.310
Rmerge	0.027	0.055
Number of reflections	30304
<I/σ(I)>	34.8	10.8
Completeness [%]	91.7	74.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	12%PEG 400,200mM NaCl, pH 7., VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 400
2	1	1	NaCl
3	1	2	PEG 400
4	1	2	NaCl