2P14

Crystal structure of small subunit (R.BspD6I2) of the heterodimeric restriction endonuclease R.BspD6I

Summary for 2P14

Related2ewf
DescriptorHeterodimeric restriction endonuclease R.BspD6I small subunit, SULFATE ION, GLYCEROL, ... (4 entities in total)
Functional Keywordsheterodimeric restriction endonuclease, hydrolase
Biological sourceBacillus sp.
Total number of polymer chains1
Total molecular weight22792.73
Authors
Kachalova, G.S.,Bartunik, H.D.,Artyukh, R.I.,Rogulin, E.A.,Yunusova, A.K.,Zheleznaya, L.A.,Matvienko, N.I. (deposition date: 2007-03-02, release date: 2008-03-11, Last modification date: 2011-07-13)
Primary citation
Kachalova, G.S.,Rogulin, E.A.,Yunusova, A.K.,Artyukh, R.I.,Perevyazova, T.A.,Matvienko, N.I.,Zheleznaya, L.A.,Bartunik, H.D.
Structural analysis of the heterodimeric type IIS restriction endonuclease R.BspD6I acting as a complex between a monomeric site-specific nickase and a catalytic subunit.
J.Mol.Biol., 384:489-502, 2008
PubMed: 18835275 (PDB entries with the same primary citation)
DOI: 10.1016/j.jmb.2008.09.033
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.5 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.210602.4%3.8%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 2p14
no rotation
Molmil generated image of 2p14
rotated about x axis by 90°
Molmil generated image of 2p14
rotated about y axis by 90°