2P14
Crystal structure of small subunit (R.BspD6I2) of the heterodimeric restriction endonuclease R.BspD6I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-17 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.429, 38.360, 61.995 |
| Unit cell angles | 90.00, 101.47, 90.00 |
Refinement procedure
| Resolution | 10.750 - 1.500 |
| R-factor | 0.179 |
| Rwork | 0.177 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ewf |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.421 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.054 | 0.470 |
| Number of reflections | 28430 | |
| <I/σ(I)> | 21.2 | 2.24 |
| Completeness [%] | 99.3 | |
| Redundancy | 2.91 | 2.87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 298 | 2.0 M (NH4)2SO4, 0.2 M Li2SO4, pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
