Structure of APPBP1-UBA3~NEDD8-NEDD8-MgATP-Ubc12(C111A), a trapped ubiquitin-like protein activation complex

Summary for 2NVU

DescriptorNEDD8-activating enzyme E1 regulatory subunit, Maltose binding protein/NEDD8-activating enzyme E1 catalytic subunit chimera, NEDD8-conjugating enzyme Ubc12, ... (8 entities in total)
Functional Keywordsmultifunction macromolecular complex, ubiquitin, nedd8, e1, e2, atp, conformational change, thioester, switch, adenylation, protein turnover, ligase
Biological sourceHomo sapiens (human)
Cellular locationCell membrane Q13564
Nucleus Q15843
Total number of polymer chains5
Total molecular weight188893.13
Huang, D.T.,Hunt, H.W.,Zhuang, M.,Ohi, M.D.,Holton, J.M.,Schulman, B.A. (deposition date: 2006-11-13, release date: 2007-01-30, Last modification date: 2011-07-13)
Primary citation
Huang, D.T.,Hunt, H.W.,Zhuang, M.,Ohi, M.D.,Holton, J.M.,Schulman, B.A.
Basis for a ubiquitin-like protein thioester switch toggling E1-E2 affinity.
Nature, 445:394-398, 2007
PubMed: 17220875 (PDB entries with the same primary citation)
DOI: 10.1038/nature05490
MImport into Mendeley
Experimental method
NMR Information

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.237211.2%5.6%12.0%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution