2LD8
Structure of Human Telomeric DNA in Crowded Solution
Summary for 2LD8
| Entry DOI | 10.2210/pdb2ld8/pdb |
| Related | 1KF1 2gku 2JSL 2kf8 2km3 |
| NMR Information | BMRB: 17655 |
| Descriptor | Human Telomeric DNA (1 entity in total) |
| Functional Keywords | g-quadruplex, propeller-type parallel-stranded, molecular crowding, dna |
| Total number of polymer chains | 1 |
| Total formula weight | 7287.69 |
| Authors | Heddi, B.,Phan, A.T. (deposition date: 2011-05-17, release date: 2011-06-29, Last modification date: 2024-05-15) |
| Primary citation | Heddi, B.,Phan, A.T. Structure of Human Telomeric DNA in Crowded Solution J.Am.Chem.Soc., 2011 Cited by PubMed Abstract: G-quadruplex structures formed by DNA at the human telomeres are attractive anticancer targets. Human telomeric sequences can adopt a diverse range of intramolecular G-quadruplex conformations: a parallel-stranded conformation was observed in the crystalline state, while at least four other forms were seen in K(+) solution, raising the question of which conformation is favored in crowded cellular environment. Here, we report the first NMR structure of a human telomeric G-quadruplex in crowded solution. We show that four different G-quadruplex conformations are converted to a propeller-type parallel-stranded G-quadruplex in K(+)-containing crowded solution due to water depletion. This study also reveals the formation of a new higher-order G-quadruplex structure under molecular crowding conditions. Our molecular dynamics simulations of solvent distribution provide insights at molecular level on the formation of parallel-stranded G-quadruplex in environment depleted of water. These results regarding human telomeric DNA can be extended to oncogenic promoters and other genomic G-rich sequences. PubMed: 21548653DOI: 10.1021/ja200786q PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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