2KZE
Structure of an all-parallel-stranded G-quadruplex formed by hTERT promoter sequence
Summary for 2KZE
| Entry DOI | 10.2210/pdb2kze/pdb |
| Related | 2A5P 2KZD 2O3M |
| Descriptor | DNA (5'-D(*AP*IP*GP*GP*GP*AP*GP*GP*GP*IP*CP*TP*GP*GP*GP*AP*GP*GP*GP*C)-3') (1 entity in total) |
| Functional Keywords | anticancer targets, g-quadruplex, htert promoter, telomerase inhibition, dna |
| Total number of polymer chains | 1 |
| Total formula weight | 6356.07 |
| Authors | Lim, K.W.,Lacroix, L.,Yue, D.J.E.,Lim, J.K.C.,Lim, J.M.W.,Phan, A.T. (deposition date: 2010-06-16, release date: 2010-10-06, Last modification date: 2024-05-01) |
| Primary citation | Lim, K.W.,Lacroix, L.,Yue, D.J.E.,Lim, J.K.C.,Lim, J.M.W.,Phan, A.T. Coexistence of two distinct G-quadruplex conformations in the hTERT promoter J.Am.Chem.Soc., 132:12331-12342, 2010 Cited by PubMed Abstract: The catalytic subunit of human telomerase, hTERT, actively elongates the 3' end of the telomere in most cancer cells. The hTERT promoter, which contains many guanine-rich stretches on the same DNA strand, exhibits an exceptional potential for G-quadruplex formation. Here we show that one particular G-rich sequence in this region coexists in two G-quadruplex conformations in potassium solution: a (3 + 1) and a parallel-stranded G-quadruplexes. We present the NMR solution structures of both conformations, each comprising several robust structural elements, among which include the (3 + 1) and all-parallel G-tetrad cores, single-residue double-chain-reversal loops, and a capping A.T base pair. A combination of NMR and CD techniques, complemented with sequence modifications and variations of experimental condition, allowed us to better understand the coexistence of the two G-quadruplex conformations in equilibrium and how different structural elements conspire to favor a particular form. PubMed: 20704263DOI: 10.1021/ja101252n PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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