2K33

Solution structure of an N-glycosylated protein using in vitro glycosylation

Summary for 2K33

• Entry DOI: 10.2210/pdb2k33/pdb
• Related: 2K32
• NMR Information: BMRB: 15737
• Descriptor: AcrA, 2-acetamido-2-deoxy-alpha-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-galactopyranose-(1-4)-[beta-D-glucopyranose-(1-3)]2-acetamido-2-deoxy-alpha-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-galactopyranose-(1-3)-2,4-bisacetamido-2,4,6-trideoxy-beta-D-glucopyranose (2 entities in total)
• Functional Keywords: glycoprotein, n-glycan, glycosylation, membrane protein, transport protein
• Biological source: Campylobacter jejuni
• Total number of polymer chains: 1
• Total formula weight: 14172.61

Authors

Slynko, V.,Schubert, M.,Numao, S.,Kowarik, M.,Aebi, M.,Allain, F.H.-T. (deposition date: 2008-04-18, release date: 2009-02-03, Last modification date: 2021-10-20)

• Primary citation: Slynko, V.,Schubert, M.,Numao, S.,Kowarik, M.,Aebi, M.,Allain, F.H.; NMR structure determination of a segmentally labeled glycoprotein using in vitro glycosylation.; J.Am.Chem.Soc., 131:1274-1281, 2009; PubMed: 19154179; DOI: 10.1021/ja808682v

Experimental method

SOLUTION NMR