2K33

Solution structure of an N-glycosylated protein using in vitro glycosylation

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Bruker	AVANCE	Bruker Avance	500
2	Bruker	AVANCE	Bruker Avance	600
3	Bruker	AVANCE	Bruker Avance	750
4	Bruker	AVANCE	Bruker Avance	900

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	1	2D 1H-15N HSQC
10	1	4	3D HCCH-TOCSY
11	1	2	3D 1H-13C NOESY
12	1	1	3D 1H-15N NOESY
13	1	4	2D 13C filtered-filtered NOESY
14	1	1	2D 15N filtered-filtered NOESY
15	1	4	3D 13C edited-filtered NOESY
16	1	4	2D 13C filtered-edited NOESY
17	1	4	2D 1H-13C HSQC
2	1	3	2D 1H-15N HSQC
3	1	2	2D 1H-13C HSQC
4	1	1	2D 1H-13C HSQC
5	1	1	2D 1H-1H NOESY
6	1	3	2D 1H-1H NOESY
7	1	2	3D CBCA(CO)NH
8	1	2	3D HNCA
9	1	2	3D HN(CO)CA

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	6.4	ambient	303

Conformers

Conformers Calculated Total Number	1000
Conformers Submitted Total Number	20