2K33

Solution structure of an N-glycosylated protein using in vitro glycosylation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	AcrA	polymer	116	12748.2	1	UniProt (Q8RTE5) Pfam (PF16576)	Campylobacter jejuni
2	B (B)	2-acetamido-2-deoxy-alpha-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-galactopyranose-(1-4)-[beta-D-glucopyranose-(1-3)]2-acetamido-2-deoxy-alpha-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-galactopyranose-(1-3)-2,4-bisacetamido-2,4,6-trideoxy-beta-D-glucopyranose	branched		1424.4	1	In PDB GlyTouCan (G66397DB)

Sequence modifications

A: 1 - 36 (UniProt: Q8RTE5)

PDB	External Database	Details
Gln 36	Lys 96	engineered mutation

A: 37 - 64 (UniProt: Q8RTE5)

PDB	External Database	Details
Gln 50	Lys 131	engineered mutation

A: 65 - 108 (UniProt: Q8RTE5)

PDB	External Database	Details
Gly 109	-	expression tag
Ser 110	-	expression tag
His 111	-	expression tag
His 112	-	expression tag
His 113	-	expression tag
His 114	-	expression tag
His 115	-	expression tag
His 116	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	12748.2
Branched	Number of molecules	1
	Total formula weight	1424.4
All*	Total formula weight	14172.6

*Water molecules are not included.