2JU4
NMR structure of the gamma subunit of cGMP phosphodiesterase
Summary for 2JU4
| Entry DOI | 10.2210/pdb2ju4/pdb |
| NMR Information | BMRB: 15430 |
| Descriptor | Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma, (3'R)-1'-oxyl-2',2',5',5'-tetramethyl-1,3'-bipyrrolidine-2,5-dione (2 entities in total) |
| Functional Keywords | intrinsic disordered protein, paramagnetic relaxation enhancement, cgmp, hydrolase, sensory transduction, vision, structural genomics, psi-2, protein structure initiative, center for eukaryotic structural genomics, cesg |
| Biological source | Bos taurus (cattle) |
| Total number of polymer chains | 1 |
| Total formula weight | 11864.97 |
| Authors | Song, J.,Guo, L.W.,Ruoho, A.E.,Markley, J.L.,Center for Eukaryotic Structural Genomics (CESG) (deposition date: 2007-08-14, release date: 2007-10-16, Last modification date: 2024-10-30) |
| Primary citation | Song, J.,Guo, L.W.,Muradov, H.,Artemyev, N.O.,Ruoho, A.E.,Markley, J.L. Intrinsically disordered gamma-subunit of cGMP phosphodiesterase encodes functionally relevant transient secondary and tertiary structure. Proc.Natl.Acad.Sci.USA, 105:1505-1510, 2008 Cited by PubMed Abstract: The retinal phosphodiesterase (PDE6) inhibitory gamma-subunit (PDEgamma) plays a central role in vertebrate phototransduction through alternate interactions with the catalytic alphabeta-subunits of PDE6 and the alpha-subunit of transducin (alpha(t)). Detailed structural analysis of PDEgamma has been hampered by its intrinsic disorder. We present here the NMR solution structure of PDEgamma, which reveals a loose fold with transient structural features resembling those seen previously in the x-ray structure of PDEgamma(46-87) when bound to alpha(t) in the transition-state complex. NMR mapping of the interaction between PDEgamma(46-87) and the chimeric PDE5/6 catalytic domain confirmed that C-terminal residues 74-87 of PDEgamma are involved in the association and demonstrated that its W70 indole group, which is critical for subsequent binding to alpha(t), is left free at this stage. These results indicate that the interaction between PDEgamma and alpha(t) during the phototransduction cascade involves the selection of preconfigured transient conformations. PubMed: 18230733DOI: 10.1073/pnas.0709558105 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
Download full validation report
