2JJS

Structure of human CD47 in complex with human signal regulatory protein (SIRP) alpha

2JJS の概要

• エントリーDOI: 10.2210/pdb2jjs/pdb
• 関連するPDBエントリー: 2JJT 2UV3 2VSC
• 分子名称: TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE SUBSTRATE 1, LEUKOCYTE SURFACE ANTIGEN CD47, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: signal regulatory protein alpha, immunoglobulin superfamily, transmembrane, phosphoprotein, paired receptor, polymorphism, glycoprotein, cell adhesion, pyrrolidone carboxylic acid, alternative splicing, immunoglobulin domain, sirp, cd47, sirpa, membrane, sh3-binding
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 60487.25

構造登録者

Hatherley, D.,Graham, S.C.,Turner, J.,Harlos, K.,Stuart, D.I.,Barclay, A.N. (登録日: 2008-04-22, 公開日: 2008-08-05, 最終更新日: 2024-10-23)

主引用文献

Hatherley, D.,Graham, S.C.,Turner, J.,Harlos, K.,Stuart, D.I.,Barclay, A.N.
Paired receptor specificity explained by structures of signal regulatory proteins alone and complexed with CD47.
Mol. Cell, 31:266-277, 2008

PubMed Abstract: CD47 is a widely distributed cell-surface protein that acts a marker of self through interactions of myeloid and neural cells. We describe the high-resolution X-ray crystallographic structures of the immunoglobulin superfamily domain of CD47 alone and in complex with the N-terminal ligand-binding domain of signal regulatory protein alpha (SIRPalpha). The unusual and convoluted interacting face of CD47, comprising the N terminus and loops at the end of the domain, intercalates with the corresponding regions in SIRPalpha. We have also determined structures of the N-terminal domains of SIRPbeta, SIRPbeta(2), and SIRPgamma; proteins that are closely related to SIRPalpha but bind CD47 with negligible or reduced affinity. These results explain the specificity of CD47 for the SIRP family of paired receptors in atomic detail. Analysis of SIRPalpha polymorphisms suggests that these, as well as the activating SIRPs, may have evolved to counteract pathogen binding to the inhibitory SIRPalpha receptor.

PubMed: 18657508
DOI: 10.1016/j.molcel.2008.05.026

実験手法

X-RAY DIFFRACTION (1.85 Å)