2JJS
Structure of human CD47 in complex with human signal regulatory protein (SIRP) alpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-15 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.477, 72.410, 89.826 |
Unit cell angles | 90.00, 95.01, 90.00 |
Refinement procedure
Resolution | 89.440 - 1.850 |
R-factor | 0.171 |
Rwork | 0.169 |
R-free | 0.21300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2UV3 FOR CHAINS A AND B PDB ENTRY 2ICC FOR CHAINS C AND D |
RMSD bond length | 0.010 |
RMSD bond angle | 1.269 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.800 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.140 | 0.790 |
Number of reflections | 46373 | |
<I/σ(I)> | 17.6 | 2.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.3 | 9.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.4 | 278 | 100 NL SIRP ALPHA / CD47 COMPLEX (1:1 RATIO, EACH PROTEIN AT APPROX. 0.375 UM) PLUS 100 NL RESERVOIR (0.2 M POTASSIUM IODIDE, 20% W/V PEG 3350) EQUILIBRATED AGAINST 95 UL OF RESERVOIR AT 5 C., pH 7.4 | |
1 | 7.4 | 278 | 100 NL SIRP ALPHA / CD47 COMPLEX (1:1 RATIO, EACH PROTEIN AT APPROX. 0.375 UM) PLUS 100 NL RESERVOIR (0.2 M POTASSIUM IODIDE, 20% W/V PEG 3350) EQUILIBRATED AGAINST 95 UL OF RESERVOIR AT 5 C., pH 7.4 |