2I9K

Engineered Extrahelical Base Destabilization Enhances Sequence Discrimination of DNA Methyltransferase M.HhaI

Summary for 2I9K

• Entry DOI: 10.2210/pdb2i9k/pdb
• Related: 2HR1 3MHT
• Descriptor: 5'-D(*T*GP*AP*TP*AP*GP*CP*GP*CP*TP*AP*TP*C)-3', Modification methylase HhaI, S-ADENOSYL-L-HOMOCYSTEINE (3 entities in total)
• Functional Keywords: phe124ala mutation in m.hhai, transferase-dna complex, transferase/dna
• Biological source: Haemophilus haemolyticus
• Total number of polymer chains: 3
• Total formula weight: 45283.71

Authors

Youngblood, B.,Shieh, F.K.,De Los Rios, S.,Perona, J.J.,Reich, N.O. (deposition date: 2006-09-05, release date: 2006-10-10, Last modification date: 2024-02-21)

• Primary citation: Youngblood, B.,Shieh, F.K.,De Los Rios, S.,Perona, J.J.,Reich, N.O.; Engineered Extrahelical Base Destabilization Enhances Sequence Discrimination of DNA Methyltransferase M.HhaI; J.Mol.Biol., 362:334-346, 2006; PubMed: 16919299; DOI: 10.1016/j.jmb.2006.07.031

Experimental method

X-RAY DIFFRACTION (2.65 Å)