2I9K
Engineered Extrahelical Base Destabilization Enhances Sequence Discrimination of DNA Methyltransferase M.HhaI
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (C, D) | 5'-D(*T*GP*AP*TP*AP*GP*CP*GP*CP*TP*AP*TP*C)-3' | polymer | 13 | 3966.6 | 2 | |||
| 2 | C (A) | Modification methylase HhaI | polymer | 327 | 36966.1 | 1 | UniProt (P05102) | Haemophilus haemolyticus | Cytosine-specific methyltransferase HhaI, M.HhaI |
| 3 | D (A) | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 1 | Chemie (SAH) |
Sequence modifications
A: 1 - 327 (UniProt: P05102)
| PDB | External Database | Details |
|---|---|---|
| Ala 124 | Phe 124 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 44899.3 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 384.4 | |
| All* | Total formula weight | 45283.7 |
