2I9K
Engineered Extrahelical Base Destabilization Enhances Sequence Discrimination of DNA Methyltransferase M.HhaI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-07-15 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.541 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 97.510, 97.510, 319.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 2.790 - 2.650 |
| R-factor | 0.287 |
| Rwork | 0.238 |
| R-free | 0.26200 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.380 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.084 | 0.390 |
| Number of reflections | 17397 | |
| <I/σ(I)> | 7.1 | 1.9 |
| Completeness [%] | 99.6 | 97.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 290 | 20% Ammonium Sulfate, 100mM Sodium Citrate buffer, pH 6.0, hanging drop, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Ammonium Sulfate | ||
| 2 | 1 | 1 | Sodium Citrate buffer | ||
| 3 | 1 | 1 | HOH | ||
| 4 | 1 | 2 | Ammonium Sulfate | ||
| 5 | 1 | 2 | Sodium Citrate buffer | ||
| 6 | 1 | 2 | HOH |
