2HXY

Crystal structure of human apo-eIF4AIII

Summary for 2HXY

• Entry DOI: 10.2210/pdb2hxy/pdb
• Related: 1fuu 2HYI
• Descriptor: Probable ATP-dependent RNA helicase DDX48 (1 entity in total)
• Functional Keywords: helicase, atpase, hydrolase
• Biological source: Homo sapiens (human)
• Cellular location: Nucleus: P38919
• Total number of polymer chains: 4
• Total formula weight: 179274.17

Authors

Johansen, J.S.,Andersen, G.R. (deposition date: 2006-08-04, release date: 2006-08-15, Last modification date: 2024-02-14)

Primary citation

Andersen, C.B.,Ballut, L.,Johansen, J.S.,Chamieh, H.,Nielsen, K.H.,Oliveira, C.L.,Pedersen, J.S.,Seraphin, B.,Le Hir, H.,Andersen, G.R.
Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA.
Science, 313:1968-1972, 2006

PubMed Abstract: In higher eukaryotes, a multiprotein exon junction complex is deposited on spliced messenger RNAs. The complex is organized around a stable core, which serves as a binding platform for numerous factors that influence messenger RNA function. Here, we present the crystal structure of a tetrameric exon junction core complex containing the DEAD-box adenosine triphosphatase (ATPase) eukaryotic initiation factor 4AIII (eIF4AIII) bound to an ATP analog, MAGOH, Y14, a fragment of MLN51, and a polyuracil mRNA mimic. eIF4AIII interacts with the phosphate-ribose backbone of six consecutive nucleotides and prevents part of the bound RNA from being double stranded. The MAGOH and Y14 subunits lock eIF4AIII in a prehydrolysis state, and activation of the ATPase probably requires only modest conformational changes in eIF4AIII motif I.

PubMed: 16931718
DOI: 10.1126/science.1131981

Experimental method

X-RAY DIFFRACTION (3.3 Å)