2HXY
Crystal structure of human apo-eIF4AIII
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Detector technology | CCD |
Collection date | 2006-05-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 150.220, 238.050, 79.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.300 |
R-factor | 0.276 |
Rwork | 0.275 |
R-free | 0.30800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.680 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.500 |
High resolution limit [Å] | 3.300 | 3.300 |
Number of reflections | 43678 | |
<I/σ(I)> | 11.4 | 2.86 |
Completeness [%] | 99.6 | 99.6 |
Redundancy | 4.88 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 277 | Ammonium Citrate, pH 7.5, VAPOR DIFFUSION, temperature 277K |