Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2HXY

Crystal structure of human apo-eIF4AIII

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000184	biological_process	nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
A	0000398	biological_process	mRNA splicing, via spliceosome
A	0003676	molecular_function	nucleic acid binding
A	0003723	molecular_function	RNA binding
A	0003724	molecular_function	RNA helicase activity
A	0003729	molecular_function	mRNA binding
A	0004386	molecular_function	helicase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005681	cellular_component	spliceosomal complex
A	0005730	cellular_component	nucleolus
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006364	biological_process	rRNA processing
A	0006397	biological_process	mRNA processing
A	0006406	biological_process	mRNA export from nucleus
A	0006417	biological_process	regulation of translation
A	0008143	molecular_function	poly(A) binding
A	0008306	biological_process	associative learning
A	0008380	biological_process	RNA splicing
A	0010629	biological_process	negative regulation of gene expression
A	0016020	cellular_component	membrane
A	0016071	biological_process	mRNA metabolic process
A	0016607	cellular_component	nuclear speck
A	0016787	molecular_function	hydrolase activity
A	0016887	molecular_function	ATP hydrolysis activity
A	0017148	biological_process	negative regulation of translation
A	0030425	cellular_component	dendrite
A	0035145	cellular_component	exon-exon junction complex
A	0035368	molecular_function	selenocysteine insertion sequence binding
A	0035613	molecular_function	RNA stem-loop binding
A	0035640	biological_process	exploration behavior
A	0043021	molecular_function	ribonucleoprotein complex binding
A	0043025	cellular_component	neuronal cell body
A	0045727	biological_process	positive regulation of translation
A	0048701	biological_process	embryonic cranial skeleton morphogenesis
A	0051028	biological_process	mRNA transport
A	0071006	cellular_component	U2-type catalytic step 1 spliceosome
A	0071013	cellular_component	catalytic step 2 spliceosome
A	0072715	biological_process	cellular response to selenite ion
A	0090394	biological_process	negative regulation of excitatory postsynaptic potential
A	0098794	cellular_component	postsynapse
A	0098978	cellular_component	glutamatergic synapse
A	0099578	biological_process	regulation of translation at postsynapse, modulating synaptic transmission
A	1904570	biological_process	negative regulation of selenocysteine incorporation
A	1990416	biological_process	cellular response to brain-derived neurotrophic factor stimulus
A	1990904	cellular_component	ribonucleoprotein complex
A	2000622	biological_process	regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
B	0000166	molecular_function	nucleotide binding
B	0000184	biological_process	nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
B	0000398	biological_process	mRNA splicing, via spliceosome
B	0003676	molecular_function	nucleic acid binding
B	0003723	molecular_function	RNA binding
B	0003724	molecular_function	RNA helicase activity
B	0003729	molecular_function	mRNA binding
B	0004386	molecular_function	helicase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005681	cellular_component	spliceosomal complex
B	0005730	cellular_component	nucleolus
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006364	biological_process	rRNA processing
B	0006397	biological_process	mRNA processing
B	0006406	biological_process	mRNA export from nucleus
B	0006417	biological_process	regulation of translation
B	0008143	molecular_function	poly(A) binding
B	0008306	biological_process	associative learning
B	0008380	biological_process	RNA splicing
B	0010629	biological_process	negative regulation of gene expression
B	0016020	cellular_component	membrane
B	0016071	biological_process	mRNA metabolic process
B	0016607	cellular_component	nuclear speck
B	0016787	molecular_function	hydrolase activity
B	0016887	molecular_function	ATP hydrolysis activity
B	0017148	biological_process	negative regulation of translation
B	0030425	cellular_component	dendrite
B	0035145	cellular_component	exon-exon junction complex
B	0035368	molecular_function	selenocysteine insertion sequence binding
B	0035613	molecular_function	RNA stem-loop binding
B	0035640	biological_process	exploration behavior
B	0043021	molecular_function	ribonucleoprotein complex binding
B	0043025	cellular_component	neuronal cell body
B	0045727	biological_process	positive regulation of translation
B	0048701	biological_process	embryonic cranial skeleton morphogenesis
B	0051028	biological_process	mRNA transport
B	0071006	cellular_component	U2-type catalytic step 1 spliceosome
B	0071013	cellular_component	catalytic step 2 spliceosome
B	0072715	biological_process	cellular response to selenite ion
B	0090394	biological_process	negative regulation of excitatory postsynaptic potential
B	0098794	cellular_component	postsynapse
B	0098978	cellular_component	glutamatergic synapse
B	0099578	biological_process	regulation of translation at postsynapse, modulating synaptic transmission
B	1904570	biological_process	negative regulation of selenocysteine incorporation
B	1990416	biological_process	cellular response to brain-derived neurotrophic factor stimulus
B	1990904	cellular_component	ribonucleoprotein complex
B	2000622	biological_process	regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
C	0000166	molecular_function	nucleotide binding
C	0000184	biological_process	nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
C	0000398	biological_process	mRNA splicing, via spliceosome
C	0003676	molecular_function	nucleic acid binding
C	0003723	molecular_function	RNA binding
C	0003724	molecular_function	RNA helicase activity
C	0003729	molecular_function	mRNA binding
C	0004386	molecular_function	helicase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005681	cellular_component	spliceosomal complex
C	0005730	cellular_component	nucleolus
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006364	biological_process	rRNA processing
C	0006397	biological_process	mRNA processing
C	0006406	biological_process	mRNA export from nucleus
C	0006417	biological_process	regulation of translation
C	0008143	molecular_function	poly(A) binding
C	0008306	biological_process	associative learning
C	0008380	biological_process	RNA splicing
C	0010629	biological_process	negative regulation of gene expression
C	0016020	cellular_component	membrane
C	0016071	biological_process	mRNA metabolic process
C	0016607	cellular_component	nuclear speck
C	0016787	molecular_function	hydrolase activity
C	0016887	molecular_function	ATP hydrolysis activity
C	0017148	biological_process	negative regulation of translation
C	0030425	cellular_component	dendrite
C	0035145	cellular_component	exon-exon junction complex
C	0035368	molecular_function	selenocysteine insertion sequence binding
C	0035613	molecular_function	RNA stem-loop binding
C	0035640	biological_process	exploration behavior
C	0043021	molecular_function	ribonucleoprotein complex binding
C	0043025	cellular_component	neuronal cell body
C	0045727	biological_process	positive regulation of translation
C	0048701	biological_process	embryonic cranial skeleton morphogenesis
C	0051028	biological_process	mRNA transport
C	0071006	cellular_component	U2-type catalytic step 1 spliceosome
C	0071013	cellular_component	catalytic step 2 spliceosome
C	0072715	biological_process	cellular response to selenite ion
C	0090394	biological_process	negative regulation of excitatory postsynaptic potential
C	0098794	cellular_component	postsynapse
C	0098978	cellular_component	glutamatergic synapse
C	0099578	biological_process	regulation of translation at postsynapse, modulating synaptic transmission
C	1904570	biological_process	negative regulation of selenocysteine incorporation
C	1990416	biological_process	cellular response to brain-derived neurotrophic factor stimulus
C	1990904	cellular_component	ribonucleoprotein complex
C	2000622	biological_process	regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
D	0000166	molecular_function	nucleotide binding
D	0000184	biological_process	nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
D	0000398	biological_process	mRNA splicing, via spliceosome
D	0003676	molecular_function	nucleic acid binding
D	0003723	molecular_function	RNA binding
D	0003724	molecular_function	RNA helicase activity
D	0003729	molecular_function	mRNA binding
D	0004386	molecular_function	helicase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005681	cellular_component	spliceosomal complex
D	0005730	cellular_component	nucleolus
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006364	biological_process	rRNA processing
D	0006397	biological_process	mRNA processing
D	0006406	biological_process	mRNA export from nucleus
D	0006417	biological_process	regulation of translation
D	0008143	molecular_function	poly(A) binding
D	0008306	biological_process	associative learning
D	0008380	biological_process	RNA splicing
D	0010629	biological_process	negative regulation of gene expression
D	0016020	cellular_component	membrane
D	0016071	biological_process	mRNA metabolic process
D	0016607	cellular_component	nuclear speck
D	0016787	molecular_function	hydrolase activity
D	0016887	molecular_function	ATP hydrolysis activity
D	0017148	biological_process	negative regulation of translation
D	0030425	cellular_component	dendrite
D	0035145	cellular_component	exon-exon junction complex
D	0035368	molecular_function	selenocysteine insertion sequence binding
D	0035613	molecular_function	RNA stem-loop binding
D	0035640	biological_process	exploration behavior
D	0043021	molecular_function	ribonucleoprotein complex binding
D	0043025	cellular_component	neuronal cell body
D	0045727	biological_process	positive regulation of translation
D	0048701	biological_process	embryonic cranial skeleton morphogenesis
D	0051028	biological_process	mRNA transport
D	0071006	cellular_component	U2-type catalytic step 1 spliceosome
D	0071013	cellular_component	catalytic step 2 spliceosome
D	0072715	biological_process	cellular response to selenite ion
D	0090394	biological_process	negative regulation of excitatory postsynaptic potential
D	0098794	cellular_component	postsynapse
D	0098978	cellular_component	glutamatergic synapse
D	0099578	biological_process	regulation of translation at postsynapse, modulating synaptic transmission
D	1904570	biological_process	negative regulation of selenocysteine incorporation
D	1990416	biological_process	cellular response to brain-derived neurotrophic factor stimulus
D	1990904	cellular_component	ribonucleoprotein complex
D	2000622	biological_process	regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay

Functional Information from PROSITE/UniProt

site_id	PS00039
Number of Residues	9
Details	DEAD_ATP_HELICASE DEAD-box subfamily ATP-dependent helicases signature. VLDEADEmL

Chain	Residue	Details
A	VAL185-LEU193

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	680
Details	Domain: {"description":"Helicase ATP-binding","evidences":[{"source":"PROSITE-ProRule","id":"PRU00541","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	644
Details	Domain: {"description":"Helicase C-terminal","evidences":[{"source":"PROSITE-ProRule","id":"PRU00542","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	112
Details	Motif: {"description":"Q motif"}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	12
Details	Motif: {"description":"DEAD box","evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	48
Details	Binding site: {"evidences":[{"source":"PubMed","id":"16923391","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16931718","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19033377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20479275","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2HYI","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2J0Q","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2J0S","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2XB2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3EX7","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	4
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P60842","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	4
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	4
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P60843","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	8
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	8
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"UniProtKB","id":"P60842","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	12
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)