2F5M
Cross-linked barnase soaked in bromo-ethanol
Summary for 2F5M
| Entry DOI | 10.2210/pdb2f5m/pdb |
| Related | 2F4Y 2F56 |
| Descriptor | Ribonuclease, 2-BROMOETHANOL (3 entities in total) |
| Functional Keywords | denaturation, lysozyme, barnase, cross-linked crystals, glutaraldehyde, urea, thiourea, bromoethanol, hydrolase |
| Biological source | Bacillus amyloliquefaciens |
| Cellular location | Secreted: P00648 |
| Total number of polymer chains | 3 |
| Total formula weight | 36848.47 |
| Authors | Prange, T.,Salem, M. (deposition date: 2005-11-26, release date: 2006-04-25, Last modification date: 2023-08-23) |
| Primary citation | Salem, M.,Mauguen, Y.,Prange, T. On the edge of the denaturation process: Application of X-ray diffraction to barnase and lysozyme cross-linked crystals with denaturants in molar concentrations. Biochim.Biophys.Acta, 1764:903-912, 2006 Cited by PubMed: 16600702DOI: 10.1016/j.bbapap.2006.02.009 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.95 Å) |
Structure validation
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