2F4Y

Barnase cross-linked with glutaraldehyde

Summary for 2F4Y

• Entry DOI: 10.2210/pdb2f4y/pdb
• Related: 1A2P
• Descriptor: Ribonuclease, ZINC ION (3 entities in total)
• Functional Keywords: denaturation, lysozyme, barnase, cross-linked crystals, glutaraldehyde, urea, thiourea, bromoethanol, hydrolase
• Biological source: Bacillus amyloliquefaciens
• Cellular location: Secreted: P00648
• Total number of polymer chains: 3
• Total formula weight: 36663.95

Authors

Prange, T.,Salem, M. (deposition date: 2005-11-24, release date: 2006-04-25, Last modification date: 2023-08-23)

Primary citation

Salem, M.,Mauguen, Y.,Prange, T.
On the edge of the denaturation process: Application of X-ray diffraction to barnase and lysozyme cross-linked crystals with denaturants in molar concentrations.
Biochim.Biophys.Acta, 1764:903-912, 2006

PubMed Abstract: Structural data about the early step of protein denaturation were obtained from cross-linked crystals for two small proteins: barnase and lysozyme. Several denaturant agents like urea, bromoethanol or thiourea were used at increasing concentrations up to a limit leading to crystal disruption (>or=2 to 6 M). Before the complete destruction of the crystal order started, specific binding sites were observed at the protein surfaces, an indication that the preliminary step of denaturation is the disproportion of intermolecular polar bonds to the benefit of the agent "parasiting" the surface. The analysis of the thermal factors first agree with a stabilization effect at low or moderate concentration of denaturants rapidly followed by a destabilization at specific weak points when the number of sites increase (overflooding effect).

PubMed: 16600702
DOI: 10.1016/j.bbapap.2006.02.009

Experimental method

X-RAY DIFFRACTION (2.15 Å)