2F4Y

Barnase cross-linked with glutaraldehyde

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	2002-03-25
Detector	MARRESEARCH
Wavelength(s)	0.964
Spacegroup name	P 32
Unit cell lengths	58.970, 58.970, 82.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	9.000 - 2.150
Rwork	0.147
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1a2p
RMSD bond length	0.005
RMSD bond angle	0.023
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	SHELXL

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9.000	2.500
High resolution limit [Å]	2.150	2.150
Number of reflections	14863
<I/σ(I)>	16.3	7.6
Completeness [%]	93.1	92.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	300	AMMONIUM SULFATE, ZINC CHLORIDE, HEPES BUFFER PROTEIN AT 20MG/ML, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 300K