2F5M
Cross-linked barnase soaked in bromo-ethanol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 2002-03-26 |
Detector | MARRESEARCH |
Wavelength(s) | 0.964 |
Spacegroup name | P 32 |
Unit cell lengths | 59.280, 59.280, 81.550 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 9.500 - 1.950 |
Rwork | 0.178 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2f4y |
RMSD bond length | 0.007 |
RMSD bond angle | 0.024 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 9.500 | 2.100 |
High resolution limit [Å] | 1.949 | 1.949 |
Number of reflections | 20696 | |
<I/σ(I)> | 20.1 | 9.7 |
Completeness [%] | 95.3 | 81.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 300 | FROM AMMONIUM SULFATE, PROTEIN =20 MG/ML, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 300K |