2E59

Crystal structure of human MD-2 in complex with lipid IVa

Summary for 2E59

• Entry DOI: 10.2210/pdb2e59/pdb
• Related: 2E56
• Descriptor: Lymphocyte antigen 96, 2-acetamido-2-deoxy-beta-D-glucopyranose, (R)-((2R,3S,4R,5R,6R)-3-HYDROXY-2-(HYDROXYMETHYL)-5-((R)-3-HYDROXYTETRADECANAMIDO)-6-(PHOSPHONOOXY)TETRAHYDRO-2H-PYRAN-4-YL) 3-HYDROXYTETRADECANOATE, ... (5 entities in total)
• Functional Keywords: innate immunity, lipid-binding, lipid binding protein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 18452.27

Authors

Ohto, U.,Satow, Y. (deposition date: 2006-12-19, release date: 2007-06-26, Last modification date: 2024-10-23)

Primary citation

Ohto, U.,Fukase, K.,Miyake, K.,Satow, Y.
Crystal structures of human MD-2 and its complex with antiendotoxic lipid IVa
Science, 316:1632-1634, 2007

PubMed Abstract: Endotoxic lipopolysaccharide (LPS) with potent immunostimulatory activity is recognized by the receptor complex of MD-2 and Toll-like receptor 4. Crystal structures of human MD-2 and its complex with the antiendotoxic tetra-acylated lipid A core of LPS have been determined at 2.0 and 2.2 angstrom resolutions, respectively. MD-2 shows a deep hydrophobic cavity sandwiched by two beta sheets, in which four acyl chains of the ligand are fully confined. The phosphorylated glucosamine moieties are located at the entrance to the cavity. These structures suggest that MD-2 plays a principal role in endotoxin recognition and provide a basis for antiseptic drug development.

PubMed: 17569869
DOI: 10.1126/science.1139111

Experimental method

X-RAY DIFFRACTION (2.21 Å)