2E2H

RNA polymerase II elongation complex at 5 mM Mg2+ with GTP

Summary for 2E2H

• Entry DOI: 10.2210/pdb2e2h/pdb
• Related: 1R9S 1R9T 1SFO 2E2I 2E2J 2NVQ 2NVS 2NVT 2NVX 2NVY 2NVZ
• Descriptor: 5'-R(*AP*UP*CP*GP*AP*GP*AP*GP*GP*A)-3', DNA-directed RNA polymerase II subunit 9, DNA-directed RNA polymerases I/II/III subunit 10, ... (16 entities in total)
• Functional Keywords: transcription, mrna, multiprotein complex, molecular machine, dna, transferase-dna-rna hybrid complex, transferase/dna-rna hybrid
• Biological source: Saccharomyces cerevisiae (baker's yeast); More
• Cellular location: Nucleus: P04050 P08518 P16370 P20434 P20436 P27999 P38902; Nucleus, nucleolus: P40422 P22139; Cytoplasm: P20435
• Total number of polymer chains: 13
• Total formula weight: 486782.36

Authors

Wang, D.,Bushnell, D.A.,Westover, K.D.,Kaplan, C.D.,Kornberg, R.D. (deposition date: 2006-11-14, release date: 2006-12-12, Last modification date: 2024-10-30)

Primary citation

Wang, D.,Bushnell, D.A.,Westover, K.D.,Kaplan, C.D.,Kornberg, R.D.
Structural basis of transcription: role of the trigger loop in substrate specificity and catalysis
Cell(Cambridge,Mass.), 127:941-954, 2006

PubMed Abstract: New structures of RNA polymerase II (pol II) transcribing complexes reveal a likely key to transcription. The trigger loop swings beneath a correct nucleoside triphosphate (NTP) in the nucleotide addition site, closing off the active center and forming an extensive network of interactions with the NTP base, sugar, phosphates, and additional pol II residues. A histidine side chain in the trigger loop, precisely positioned by these interactions, may literally "trigger" phosphodiester bond formation. Recognition and catalysis are thus coupled, ensuring the fidelity of transcription.

PubMed: 17129781
DOI: 10.1016/j.cell.2006.11.023

Experimental method

X-RAY DIFFRACTION (3.95 Å)