2E2H
RNA polymerase II elongation complex at 5 mM Mg2+ with GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Collection date | 2006-05-08 |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 171.108, 222.005, 195.163 |
| Unit cell angles | 90.00, 102.61, 90.00 |
Refinement procedure
| Resolution | 45.080 - 3.950 |
| R-factor | 0.29421 |
| Rwork | 0.290 |
| R-free | 0.36755 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sfo |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.261 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.090 |
| High resolution limit [Å] | 3.950 | 3.950 |
| Number of reflections | 60760 | |
| <I/σ(I)> | 10.3 | 2.3 |
| Completeness [%] | 98.3 | 92.2 |
| Redundancy | 3.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 50mM dioxane, 10mM DTT, 9-11% PEG6000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | (NH4)2HPO4 | ||
| 2 | 1 | 1 | NaH2PO4 | ||
| 3 | 1 | 1 | dioxane | ||
| 4 | 1 | 1 | DTT | ||
| 5 | 1 | 1 | PEG6000 | ||
| 6 | 1 | 2 | (NH4)2HPO4 | ||
| 7 | 1 | 2 | NaH2PO4 | ||
| 8 | 1 | 2 | PEG6000 |
