RNA polymerase II elongation complex in 5 mM Mg+2 with GMPCPP

Summary for 2E2J

Related1SFO 1R9S 1R9T 2E2H 2E2I 2NVQ 2NVS 2NVT 2NVX 2NVY 2NVZ
Descriptor5'-R(P*AP*UP*CP*GP*AP*GP*AP*GP*G)-3', 27-MER DNA template strand, 5'-D(P*CP*TP*GP*CP*TP*TP*AP*TP*CP*GP*GP*TP*A)-3', ... (16 entities in total)
Functional Keywordstranscription, mrna, multiprotein complex, molecular machine, dna, transcription-dna-rna complex, transferase-dna-rna hybrid complex, transferase/dna-rna hybrid
Biological sourceSaccharomyces cerevisiae (baker's yeast)
Total number of polymer chains13
Total molecular weight485841.81
Wang, D.,Bushnell, D.A.,Westover, K.D.,Kaplan, C.D.,Kornberg, R.D. (deposition date: 2006-11-14, release date: 2006-12-12, Last modification date: 2019-12-04)
Primary citation
Wang, D.,Bushnell, D.A.,Westover, K.D.,Kaplan, C.D.,Kornberg, R.D.
Structural basis of transcription: role of the trigger loop in substrate specificity and catalysis
Cell(Cambridge,Mass.), 127:941-954, 2006
PubMed: 17129781 (PDB entries with the same primary citation)
DOI: 10.1016/j.cell.2006.11.023
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone 0.29318 4.3% 11.1% 1.3% 0.39MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
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PDB entries from 2020-10-21