2E2J

RNA polymerase II elongation complex in 5 mM Mg+2 with GMPCPP

Summary for 2E2J

Related1SFO 1R9S 1R9T 2E2H 2E2I 2NVQ 2NVS 2NVT 2NVX 2NVY 2NVZ
Descriptor5'-R(P*AP*UP*CP*GP*AP*GP*AP*GP*G)-3', 27-MER DNA template strand, 5'-D(P*CP*TP*GP*CP*TP*TP*AP*TP*CP*GP*GP*TP*A)-3', ... (16 entities in total)
Functional Keywordstranscription, mrna, multiprotein complex, molecular machine, dna, transcription-dna-rna complex, transferase-dna-rna hybrid complex, transferase/dna-rna hybrid
Biological sourceSaccharomyces cerevisiae (baker's yeast)
Cellular locationNucleus P04050 P08518 P16370 P20434 P20436 P27999 P38902
Cytoplasm P20435
Nucleus, nucleolus P22139 P40422
Total number of polymer chains13
Total molecular weight485841.81
Authors
Wang, D.,Bushnell, D.A.,Westover, K.D.,Kaplan, C.D.,Kornberg, R.D. (deposition date: 2006-11-14, release date: 2006-12-12, Last modification date: 2011-07-13)
Primary citation
Wang, D.,Bushnell, D.A.,Westover, K.D.,Kaplan, C.D.,Kornberg, R.D.
Structural basis of transcription: role of the trigger loop in substrate specificity and catalysis
Cell(Cambridge,Mass.), 127:941-954, 2006
PubMed: 17129781 (PDB entries with the same primary citation)
DOI: 10.1016/j.cell.2006.11.023
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (3.5 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone0.293184.3%11.1%1.3%0.39MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution