2CLT

Crystal structure of the active form (full-length) of human fibroblast collagenase.

2CLT の概要

• エントリーDOI: 10.2210/pdb2clt/pdb
• 関連するPDBエントリー: 1AYK 1CGE 1CGF 1CGL 1HFC 1SU3 2AYK 2TCL 3AYK 4AYK 966C
• 分子名称: INTERSTITIAL COLLAGENASE, CALCIUM ION, ZINC ION, ... (4 entities in total)
• 機能のキーワード: extracellular matrix, fibroblast collagenase, zinc, calcium, zymogen, protease, collagen, autocatalytic cleavage, matrix metalloproteinases, hydrolase, polymorphism, glycoprotein, metal-binding, inhibitor-free, metalloprotease, collagen degradation
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Secreted, extracellular space, extracellular matrix (Probable): P03956
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 85042.40

構造登録者

Iyer, S.,Visse, R.,Nagase, H.,Acharya, K.R. (登録日: 2006-04-29, 公開日: 2006-08-09, 最終更新日: 2024-11-06)

主引用文献

Iyer, S.,Visse, R.,Nagase, H.,Acharya, K.R.
Crystal Structure of an Active Form of Human Mmp-1.
J.Mol.Biol., 362:78-, 2006

PubMed Abstract: The extracellular matrix is a dynamic environment that constantly undergoes remodelling and degradation during vital physiological processes such as angiogenesis, wound healing, and development. Unbalanced extracellular matrix breakdown is associated with many diseases such as arthritis, cancer and fibrosis. Interstitial collagen is degraded by matrix metalloproteinases with collagenolytic activity by MMP-1, MMP-8 and MMP-13, collectively known as the collagenases. Matrix metalloproteinase 1 (MMP-1) plays a pivotal role in degradation of interstitial collagen types I, II, and III. Here, we report the crystal structure of the active form of human MMP-1 at 2.67 A resolution. This is the first MMP-1 structure that is free of inhibitor and a water molecule essential for peptide hydrolysis is observed coordinated with the active site zinc. Comparing this structure with the human proMMP-1 shows significant structural differences, mainly in the relative orientation of the hemopexin domain, between the pro form and active form of the human enzyme.

PubMed: 16890240
DOI: 10.1016/J.JMB.2006.06.079

実験手法

X-RAY DIFFRACTION (2.67 Å)