2ANR
Crystal structure (II) of Nova-1 KH1/KH2 domain tandem with 25nt RNA hairpin
Summary for 2ANR
Entry DOI | 10.2210/pdb2anr/pdb |
Related | 2ANN |
Descriptor | 5'-R(*CP*(5BU)P*CP*GP*CP*GP*GP*AP*UP*CP*AP*GP*UP*CP*AP*CP*CP*CP*AP*AP*GP*CP*GP*AP*G)-3', RNA-binding protein Nova-1, POTASSIUM ION, ... (5 entities in total) |
Functional Keywords | protein-rna complex, kh domain, hairpin, rna-binding protein-rna complex, rna-binding protein/rna |
Biological source | Mus musculus (Mouse) More |
Total number of polymer chains | 2 |
Total formula weight | 27461.29 |
Authors | Malinina, L.,Teplova, M.,Musunuru, K.,Teplov, A.,Darnell, J.C.,Burley, S.K.,Darnell, R.B.,Patel, D.J. (deposition date: 2005-08-11, release date: 2006-10-24, Last modification date: 2024-10-30) |
Primary citation | Teplova, M.,Malinina, L.,Darnell, J.C.,Song, J.,Lu, M.,Abagyan, R.,Musunuru, K.,Teplov, A.,Burley, S.K.,Darnell, R.B.,Patel, D.J. Protein-RNA and protein-protein recognition by dual KH1/2 domains of the neuronal splicing factor Nova-1. Structure, 19:930-944, 2011 Cited by PubMed Abstract: Nova onconeural antigens are neuron-specific RNA-binding proteins implicated in paraneoplastic opsoclonus-myoclonus-ataxia (POMA) syndrome. Nova harbors three K-homology (KH) motifs implicated in alternate splicing regulation of genes involved in inhibitory synaptic transmission. We report the crystal structure of the first two KH domains (KH1/2) of Nova-1 bound to an in vitro selected RNA hairpin, containing a UCAG-UCAC high-affinity binding site. Sequence-specific intermolecular contacts in the complex involve KH1 and the second UCAC repeat, with the RNA scaffold buttressed by interactions between repeats. Whereas the canonical RNA-binding surface of KH2 in the above complex engages in protein-protein interactions in the crystalline state, the individual KH2 domain can sequence-specifically target the UCAC RNA element in solution. The observed antiparallel alignment of KH1 and KH2 domains in the crystal structure of the complex generates a scaffold that could facilitate target pre-mRNA looping on Nova binding, thereby potentially explaining Nova's functional role in splicing regulation. PubMed: 21742260DOI: 10.1016/j.str.2011.05.002 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.94 Å) |
Structure validation
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