2ANR
Crystal structure (II) of Nova-1 KH1/KH2 domain tandem with 25nt RNA hairpin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-07 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.919826 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 159.453, 37.885, 38.523 |
Unit cell angles | 90.00, 96.64, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.940 |
R-factor | 0.23 |
Rwork | 0.227 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ann |
RMSD bond length | 0.013 |
RMSD bond angle | 1.682 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.600 | 2.010 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.114 | 0.464 |
Number of reflections | 16720 | |
Completeness [%] | 92.5 | 89.6 |
Redundancy | 6.55 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.6 | 293 | PEG 4000, isopropanol, potassium cloride, magnesium cloride, pH 5.6, VAPOR DIFFUSION, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 4000 | ||
2 | 1 | 1 | isopropanol | ||
3 | 1 | 1 | potassium chloride | ||
4 | 1 | 1 | magnesium chloride | ||
5 | 1 | 1 | H2O | ||
6 | 1 | 2 | PEG 4000 | ||
7 | 1 | 2 | potassium chloride | ||
8 | 1 | 2 | magnesium chloride |