2A59

Structure of 6,7-Dimethyl-8-ribityllumazine synthase from Schizosaccharomyces pombe mutant W27Y with bound ligand 5-nitroso-6-ribitylamino-2,4(1H,3H)-pyrimidinedione

Summary for 2A59

• Entry DOI: 10.2210/pdb2a59/pdb
• Related: 1KYV 1KYY 2A57 2A58
• Descriptor: 6,7-dimethyl-8-ribityllumazine synthase, PHOSPHATE ION, 5-NITROSO-6-RIBITYL-AMINO-2,4(1H,3H)-PYRIMIDINEDIONE, ... (4 entities in total)
• Functional Keywords: 6, 7-dimethyl-8-ribityllumazine synthase, schizosaccharomyces pombe, mutant w27y, lusy, 5-nitroso-6-ribitylamino-2, 4(1h, 3h)-pyrimidinedione, transferase
• Biological source: Schizosaccharomyces pombe (fission yeast)
• Total number of polymer chains: 5
• Total formula weight: 87844.78

Authors

Koch, M.,Breithaupt, C.,Gerhardt, S.,Haase, I.,Weber, S.,Cushman, M.,Huber, R.,Bacher, A.,Fischer, M. (deposition date: 2005-06-30, release date: 2005-07-19, Last modification date: 2024-02-14)

Primary citation

Koch, M.,Breithaupt, C.,Gerhardt, S.,Haase, I.,Weber, S.,Cushman, M.,Huber, R.,Bacher, A.,Fischer, M.
Structural basis of charge transfer complex formation by riboflavin bound to 6,7-dimethyl-8-ribityllumazine synthase
Eur.J.Biochem., 271:3208-3214, 2004

PubMed Abstract: The amino acid residue tryptophan 27 of 6,7-dimethyl-8-ribityllumazine synthase of the yeast Schizosaccharomyces pombe was replaced by tyrosine. The structures of the W27Y mutant protein in complex with riboflavin, the substrate analogue 5-nitroso-6-ribitylamino-2,4(1H,3H)-pyrimidinedione, and the product analogue 6-carboxyethyl-7-oxo-8-ribityllumazine, were determined by X-ray crystallography at resolutions of 2.7-2.8 A. Whereas the indole system of W27 forms a coplanar pi-complex with riboflavin, the corresponding phenyl ring in the W27Y mutant establishes only peripheral contact with the heterocyclic ring system of the bound riboflavin. These findings provide an explanation for the absence of the long wavelength shift in optical absorption spectra of riboflavin bound to the mutant enzyme. The structures of the mutants are important tools for the interpretation of the unusual physical properties of riboflavin in complex with lumazine synthase.

PubMed: 15265040
DOI: 10.1111/j.1432-1033.2004.04253.x

Experimental method

X-RAY DIFFRACTION (2.7 Å)