2QFA

Crystal structure of a Survivin-Borealin-INCENP core complex

Summary for 2QFA

• Entry DOI: 10.2210/pdb2qfa/pdb
• Descriptor: Baculoviral IAP repeat-containing protein 5, Borealin, Inner centromere protein, ... (6 entities in total)
• Functional Keywords: three-helical-bundle, long helix, protein complex, alternative splicing, apoptosis, cell cycle, cell division, centromere, chromosomal protein, cytoplasm, metal-binding, mitosis, nucleus, phosphorylation, polymorphism, protease inhibitor, thiol protease inhibitor, zinc, coiled coil, microtubule, cell cycle-cell cycle-cell cycle complex, cell cycle/cell cycle/cell cycle
• Biological source: Homo sapiens (human); More
• Cellular location: Cytoplasm: O15392; Nucleus, nucleolus: Q53HL2; Chromosome, centromere: Q9NQS7
• Total number of polymer chains: 3
• Total formula weight: 29870.69

Authors

Jeyaprakash, A.A.,Klein, U.R.,Lindner, D.,Ebert, J.,Nigg, E.A.,Conti, E. (deposition date: 2007-06-27, release date: 2007-11-06, Last modification date: 2024-02-21)

Primary citation

Jeyaprakash, A.A.,Klein, U.R.,Lindner, D.,Ebert, J.,Nigg, E.A.,Conti, E.
Structure of a Survivin-Borealin-INCENP Core Complex Reveals How Chromosomal Passengers Travel Together.
Cell(Cambridge,Mass.), 131:271-285, 2007

PubMed Abstract: The chromosomal passenger complex (CPC) is a key regulator of chromosome segregation and cytokinesis. CPC functions are connected to its localization. The complex first localizes to centromeres and later associates with the central spindle and midbody. Survivin, Borealin, and INCENP are the three components of the CPC that regulate the activity and localization of its enzymatic component, the kinase Aurora B. We determined the 1.4 A resolution crystal structure of the regulatory core of the CPC, revealing that Borealin and INCENP associate with the helical domain of Survivin to form a tight three-helical bundle. We used siRNA rescue experiments with structure-based mutants to explore the requirements for CPC localization. We show that the intertwined structural interactions of the core components lead to functional interdependence. Association of the core "passenger" proteins creates a single structural unit, whose composite molecular surface presents conserved residues essential for central spindle and midbody localization.

PubMed: 17956729
DOI: 10.1016/j.cell.2007.07.045

Experimental method

X-RAY DIFFRACTION (1.4 Å)