2QFA

Crystal structure of a Survivin-Borealin-INCENP core complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Wavelength(s)	0.99
Spacegroup name	P 21 21 21
Unit cell lengths	42.467, 78.793, 100.017
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.000 - 1.400
R-factor	0.186
Rwork	0.184
R-free	0.20200
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.326
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELXS
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.000	1.480
High resolution limit [Å]	1.400	1.400
Rmerge	0.060	0.392
Number of reflections	64114
<I/σ(I)>	15.2	2.6
Completeness [%]	96.0	80.1
Redundancy	5.1	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	293	50mM MES pH6, 10% PEG3350, VAPOR DIFFUSION, temperature 293K