2A1T

Structure of the human MCAD:ETF E165betaA complex

Summary for 2A1T

• Entry DOI: 10.2210/pdb2a1t/pdb
• Related: 1T9G 2A1U
• Descriptor: Acyl-CoA dehydrogenase, medium-chain specific, mitochondrial precursor, Electron transfer flavoprotein alpha-subunit, mitochondrial precursor, Electron transfer flavoprotein beta-subunit, ... (6 entities in total)
• Functional Keywords: electron transfer, domain dynamics, conformational sampling, protein:protein complex, fatty acid b-degradation, oxidoreductase-electron transport complex, oxidoreductase/electron transport
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 6
• Total formula weight: 253817.18

Authors

Toogood, H.S.,Van Thiel, A.,Scrutton, N.S.,Leys, D. (deposition date: 2005-06-21, release date: 2005-07-05, Last modification date: 2023-10-25)

Primary citation

Toogood, H.S.,van Thiel, A.,Scrutton, N.S.,Leys, D.
Stabilization of Non-productive Conformations Underpins Rapid Electron Transfer to Electron-transferring Flavoprotein
J.Biol.Chem., 280:30361-30366, 2005

PubMed Abstract: Crystal structures of protein complexes with electron-transferring flavoprotein (ETF) have revealed a dual protein-protein interface with one region serving as anchor while the ETF FAD domain samples available space within the complex. We show that mutation of the conserved Glu-165beta in human ETF leads to drastically modulated rates of interprotein electron transfer with both medium chain acyl-CoA dehydrogenase and dimethylglycine dehydrogenase. The crystal structure of free E165betaA ETF is essentially identical to that of wild-type ETF, but the crystal structure of the E165betaA ETF.medium chain acyl-CoA dehydrogenase complex reveals clear electron density for the FAD domain in a position optimal for fast interprotein electron transfer. Based on our observations, we present a dynamic multistate model for conformational sampling that for the wild-type ETF. medium chain acyl-CoA dehydrogenase complex involves random motion between three distinct positions for the ETF FAD domain. ETF Glu-165beta plays a key role in stabilizing positions incompatible with fast interprotein electron transfer, thus ensuring high rates of complex dissociation.

PubMed: 15975918
DOI: 10.1074/jbc.M505562200

Experimental method

X-RAY DIFFRACTION (2.8 Å)