2A1T

Structure of the human MCAD:ETF E165betaA complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001889	biological_process	liver development
A	0003995	molecular_function	acyl-CoA dehydrogenase activity
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005759	cellular_component	mitochondrial matrix
A	0005978	biological_process	glycogen biosynthetic process
A	0006082	biological_process	organic acid metabolic process
A	0006111	biological_process	regulation of gluconeogenesis
A	0006631	biological_process	fatty acid metabolic process
A	0006635	biological_process	fatty acid beta-oxidation
A	0007507	biological_process	heart development
A	0009409	biological_process	response to cold
A	0009437	biological_process	carnitine metabolic process
A	0009791	biological_process	post-embryonic development
A	0016491	molecular_function	oxidoreductase activity
A	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
A	0019254	biological_process	carnitine metabolic process, CoA-linked
A	0030424	cellular_component	axon
A	0031966	cellular_component	mitochondrial membrane
A	0033539	biological_process	fatty acid beta-oxidation using acyl-CoA dehydrogenase
A	0042594	biological_process	response to starvation
A	0042802	molecular_function	identical protein binding
A	0045329	biological_process	carnitine biosynthetic process
A	0050660	molecular_function	flavin adenine dinucleotide binding
A	0051791	biological_process	medium-chain fatty acid metabolic process
A	0051793	biological_process	medium-chain fatty acid catabolic process
A	0055007	biological_process	cardiac muscle cell differentiation
A	0070991	molecular_function	medium-chain fatty acyl-CoA dehydrogenase activity
B	0001889	biological_process	liver development
B	0003995	molecular_function	acyl-CoA dehydrogenase activity
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005759	cellular_component	mitochondrial matrix
B	0005978	biological_process	glycogen biosynthetic process
B	0006082	biological_process	organic acid metabolic process
B	0006111	biological_process	regulation of gluconeogenesis
B	0006631	biological_process	fatty acid metabolic process
B	0006635	biological_process	fatty acid beta-oxidation
B	0007507	biological_process	heart development
B	0009409	biological_process	response to cold
B	0009437	biological_process	carnitine metabolic process
B	0009791	biological_process	post-embryonic development
B	0016491	molecular_function	oxidoreductase activity
B	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
B	0019254	biological_process	carnitine metabolic process, CoA-linked
B	0030424	cellular_component	axon
B	0031966	cellular_component	mitochondrial membrane
B	0033539	biological_process	fatty acid beta-oxidation using acyl-CoA dehydrogenase
B	0042594	biological_process	response to starvation
B	0042802	molecular_function	identical protein binding
B	0045329	biological_process	carnitine biosynthetic process
B	0050660	molecular_function	flavin adenine dinucleotide binding
B	0051791	biological_process	medium-chain fatty acid metabolic process
B	0051793	biological_process	medium-chain fatty acid catabolic process
B	0055007	biological_process	cardiac muscle cell differentiation
B	0070991	molecular_function	medium-chain fatty acyl-CoA dehydrogenase activity
C	0001889	biological_process	liver development
C	0003995	molecular_function	acyl-CoA dehydrogenase activity
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005759	cellular_component	mitochondrial matrix
C	0005978	biological_process	glycogen biosynthetic process
C	0006082	biological_process	organic acid metabolic process
C	0006111	biological_process	regulation of gluconeogenesis
C	0006631	biological_process	fatty acid metabolic process
C	0006635	biological_process	fatty acid beta-oxidation
C	0007507	biological_process	heart development
C	0009409	biological_process	response to cold
C	0009437	biological_process	carnitine metabolic process
C	0009791	biological_process	post-embryonic development
C	0016491	molecular_function	oxidoreductase activity
C	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
C	0019254	biological_process	carnitine metabolic process, CoA-linked
C	0030424	cellular_component	axon
C	0031966	cellular_component	mitochondrial membrane
C	0033539	biological_process	fatty acid beta-oxidation using acyl-CoA dehydrogenase
C	0042594	biological_process	response to starvation
C	0042802	molecular_function	identical protein binding
C	0045329	biological_process	carnitine biosynthetic process
C	0050660	molecular_function	flavin adenine dinucleotide binding
C	0051791	biological_process	medium-chain fatty acid metabolic process
C	0051793	biological_process	medium-chain fatty acid catabolic process
C	0055007	biological_process	cardiac muscle cell differentiation
C	0070991	molecular_function	medium-chain fatty acyl-CoA dehydrogenase activity
D	0001889	biological_process	liver development
D	0003995	molecular_function	acyl-CoA dehydrogenase activity
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005759	cellular_component	mitochondrial matrix
D	0005978	biological_process	glycogen biosynthetic process
D	0006082	biological_process	organic acid metabolic process
D	0006111	biological_process	regulation of gluconeogenesis
D	0006631	biological_process	fatty acid metabolic process
D	0006635	biological_process	fatty acid beta-oxidation
D	0007507	biological_process	heart development
D	0009409	biological_process	response to cold
D	0009437	biological_process	carnitine metabolic process
D	0009791	biological_process	post-embryonic development
D	0016491	molecular_function	oxidoreductase activity
D	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
D	0019254	biological_process	carnitine metabolic process, CoA-linked
D	0030424	cellular_component	axon
D	0031966	cellular_component	mitochondrial membrane
D	0033539	biological_process	fatty acid beta-oxidation using acyl-CoA dehydrogenase
D	0042594	biological_process	response to starvation
D	0042802	molecular_function	identical protein binding
D	0045329	biological_process	carnitine biosynthetic process
D	0050660	molecular_function	flavin adenine dinucleotide binding
D	0051791	biological_process	medium-chain fatty acid metabolic process
D	0051793	biological_process	medium-chain fatty acid catabolic process
D	0055007	biological_process	cardiac muscle cell differentiation
D	0070991	molecular_function	medium-chain fatty acyl-CoA dehydrogenase activity
R	0005515	molecular_function	protein binding
R	0005739	cellular_component	mitochondrion
R	0005759	cellular_component	mitochondrial matrix
R	0009055	molecular_function	electron transfer activity
R	0009063	biological_process	amino acid catabolic process
R	0016491	molecular_function	oxidoreductase activity
R	0022904	biological_process	respiratory electron transport chain
R	0033539	biological_process	fatty acid beta-oxidation using acyl-CoA dehydrogenase
R	0045251	cellular_component	electron transfer flavoprotein complex
R	0050660	molecular_function	flavin adenine dinucleotide binding
S	0005515	molecular_function	protein binding
S	0005739	cellular_component	mitochondrion
S	0005759	cellular_component	mitochondrial matrix
S	0009055	molecular_function	electron transfer activity
S	0009063	biological_process	amino acid catabolic process
S	0022904	biological_process	respiratory electron transport chain
S	0033539	biological_process	fatty acid beta-oxidation using acyl-CoA dehydrogenase
S	0045251	cellular_component	electron transfer flavoprotein complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	15
Details	BINDING SITE FOR RESIDUE AMP S 600

Chain	Residue
S	ALA9
S	ALA126
S	ASP129
S	CYS131
S	ASN132
S	GLN133
S	THR134
S	VAL10
S	LYS11
S	ASN39
S	CYS42
S	CYS66
S	LEU122
S	GLY123
S	GLN125

---

site_id	AC2
Number of Residues	25
Details	BINDING SITE FOR RESIDUE FAD A 399

Chain	Residue
A	TYR133
A	VAL135
A	THR136
A	GLY141
A	SER142
A	TRP166
A	ILE167
A	THR168
A	ILE371
A	ILE374
A	TYR375
A	GLU376
A	THR378
A	GLN380
A	LEU384
A	HOH412
B	ARG281
B	THR283
B	PHE284
B	LEU288
B	HIS291
B	GLN349
B	ILE350
B	GLY353
D	GLN292

---

site_id	AC3
Number of Residues	21
Details	BINDING SITE FOR RESIDUE FAD B 1399

Chain	Residue
A	ARG281
A	THR283
A	PHE284
A	LEU288
A	HIS291
A	ILE294
A	GLN349
A	ILE350
A	GLY353
B	TYR133
B	VAL135
B	THR136
B	GLY141
B	SER142
B	TRP166
B	THR168
B	ILE371
B	TYR375
B	THR378
B	GLN380
C	GLN292

---

site_id	AC4
Number of Residues	22
Details	BINDING SITE FOR RESIDUE FAD C 2399

Chain	Residue
B	GLN292
C	TYR133
C	VAL135
C	THR136
C	GLY141
C	SER142
C	TRP166
C	ILE167
C	THR168
C	ASN214
C	ILE371
C	ILE374
C	THR378
C	GLN380
D	ARG281
D	THR283
D	PHE284
D	LEU288
D	HIS291
D	GLN349
D	ILE350
D	GLY353

---

site_id	AC5
Number of Residues	23
Details	BINDING SITE FOR RESIDUE FAD D 3399

Chain	Residue
D	THR168
D	ILE371
D	ILE374
D	TYR375
D	THR378
D	GLN380
D	HOH3402
A	GLN292
C	ARG281
C	THR283
C	PHE284
C	LEU288
C	HIS291
C	ILE294
C	GLN349
C	ILE350
C	GLY353
D	TYR133
D	VAL135
D	THR136
D	GLY141
D	SER142
D	TRP166

---

site_id	AC6
Number of Residues	30
Details	BINDING SITE FOR RESIDUE FAD R 599

Chain	Residue
C	GLN163
C	MET165
D	ASN357
R	GLY222
R	ARG223
R	GLY224
R	LYS226
R	SER248
R	ARG249
R	ALA250
R	GLN262
R	VAL263
R	GLY264
R	GLN265
R	THR266
R	GLY267
R	GLY279
R	ILE280
R	SER281
R	ALA283
R	GLN285
R	HIS286
R	ASN300
R	LYS301
R	ASP302
R	ALA317
R	ASP318
R	LEU319
R	PHE320
R	HOH602

---

Functional Information from PROSITE/UniProt

site_id	PS00072
Number of Residues	13
Details	ACYL_COA_DH_1 Acyl-CoA dehydrogenases signature 1. CVTEpgAGSDvaG

Chain	Residue	Details
A	CYS134-GLY146

---

site_id	PS00073
Number of Residues	20
Details	ACYL_COA_DH_2 Acyl-CoA dehydrogenases signature 2. QiLGGnGFntEypveKlmrD

Chain	Residue	Details
A	GLN349-ASP368

---

site_id	PS00696
Number of Residues	27
Details	ETF_ALPHA Electron transfer flavoprotein alpha-subunit signature. LYIAvGISGaIQHlaGmkdsktIvAIN

Chain	Residue	Details
R	LEU274-ASN300

---

site_id	PS01065
Number of Residues	21
Details	ETF_BETA Electron transfer flavoprotein beta-subunit signature. VeRaiDGGl.EtLrlklPaVVT

Chain	Residue	Details
S	VAL162-THR182

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:15159392, ECO:0000269|PubMed:15975918, ECO:0000269|PubMed:8962055, ECO:0007744|PDB:1EFV, ECO:0007744|PDB:1T9G, ECO:0007744|PDB:2A1T

Chain	Residue	Details
S	ALA9
S	ASN39
S	CYS66
S	GLY123
R	ASN300
R	ASP318

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: N-acetylalanine => ECO:0007744|PubMed:25944712

Chain	Residue	Details
S	ALA2
D	TYR133
D	TRP166
D	HIS291
R	LYS69
R	LYS85
R	LYS158
R	LYS203
R	LYS226
R	LYS232
C	TRP166
C	HIS291

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: N6-methyllysine; alternate => ECO:0007744|PubMed:24129315

Chain	Residue	Details
S	LYS200
C	ASP253
C	ARG256
C	GLU376
D	SER142
D	ASP253
D	ARG256
D	GLU376
R	LYS75
R	LYS101
R	LYS139
R	LYS164
B	ASP253
B	ARG256
B	GLU376
C	SER142

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: N6,N6,N6-trimethyllysine; by ETFBKMT => ECO:0000269|PubMed:25023281, ECO:0000269|PubMed:25416781

Chain	Residue	Details
S	LYS203
A	GLN349
B	ARG281
B	GLN349
C	ARG281
C	GLN349
D	ARG281
D	GLN349

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:Q9DCW4

Chain	Residue	Details
S	LYS210
B	LYS246
C	LYS44
C	LYS187
C	LYS192
C	LYS234
C	LYS246
D	LYS44
D	LYS187
D	LYS192
D	LYS234
S	LYS248
D	LYS246
A	LYS192
A	LYS234
A	LYS246
B	LYS44
B	LYS187
B	LYS192
B	LYS234

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:24275569

Chain	Residue	Details
S	SER223
S	SER226
R	LYS301
D	LYS154

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:Q9DCW4

Chain	Residue	Details
S	LYS238
A	LYS276
B	LYS254
B	LYS276
C	LYS254
C	LYS276
D	LYS254
D	LYS276

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P45952

Chain	Residue	Details
A	THR326
B	THR326
C	THR326
D	THR326

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1ivh

Chain	Residue	Details
A	THR255

---

site_id	CSA2
Number of Residues	1
Details	Annotated By Reference To The Literature 1ivh

Chain	Residue	Details
B	THR255

---

site_id	CSA3
Number of Residues	1
Details	Annotated By Reference To The Literature 1ivh

Chain	Residue	Details
C	THR255

---

site_id	CSA4
Number of Residues	1
Details	Annotated By Reference To The Literature 1ivh

Chain	Residue	Details
D	THR255

---

site_id	CSA5
Number of Residues	1
Details	Annotated By Reference To The Literature 1ivh

Chain	Residue	Details
A	GLU376

---

site_id	CSA6
Number of Residues	1
Details	Annotated By Reference To The Literature 1ivh

Chain	Residue	Details
B	GLU376

---

site_id	CSA7
Number of Residues	1
Details	Annotated By Reference To The Literature 1ivh

Chain	Residue	Details
C	GLU376

---

site_id	CSA8
Number of Residues	1
Details	Annotated By Reference To The Literature 1ivh

Chain	Residue	Details
D	GLU376

---